血清miRNAs在局灶节段性肾小球硬化及微小病变性肾病中的表达

The serum levels of miRNAs in children with focal segmental glomerulosclerosis and children with minimal change disease

目的研究局灶节段性肾小球硬化(FSGS)及微小病变性肾病(MCD)患儿miRNA的表达水平,及其与尿蛋白水平的相关性。方法选取60例经肾活检及临床确诊为FSGS(30例)、MCD(30例)的患儿,以及年龄、性别相匹配的20例正常对照儿童,用实时荧光定量PCR检测血清miR-192、miR-205水平,同时收集FSGS、MCD患儿的24 h尿蛋白及其他生化指标。结果 FSGS患儿血清miR-192、miR205表达水平明显高于MCD患儿及正常对照儿童,且与尿蛋白水平呈正相关;MCD患儿miR-205表达水平与正常对照儿童差异无统计学意义。结论 FSGS患儿血清miR-192、miR-205表达显著上调,有望作为FSGS诊断的生物标记物。

Objectives To detect the serum levels ofmiRNAs in children with focal segmental glomerulosclerosis （FSGS） and minimal change disease （MCD）, and to study its association with the level of urine protein. Methods There were three groups in the study, 30 children diagnosed of FSGS by renal biopsy, 30 children diagnosed of MCD by renal biopsy and 20 sex- and age- matched healthy controls. The serum levels of miR-192 and miR-205 were detected using real-time fluorescent quan- titative PCR. Meanwhile, 24-hour urine protein and other biochemical indices were determined. Results The serum levels of miR-192 and miR-205 in children with FSGS were significantly higher than those in the other two groups, and were positively correlated with the level of urine protein. Nevertheless, there was no difference in the serum level of miR-205 between MCD and healthy children. Conclusions The miR-192 and miR-205 in children with focal segmental glomerulosclerosis were highly up-regulated, and may be served as potential diagnostic biomarkers of FSGS.