摘要
目的探讨急性冠状动脉综合征(ACS)患者外周血辅助性T细胞(Th)17、Th17/Th1比例及血浆中白细胞介素17(IL-17)和IL-22的变化及意义。方法选择冠心病患者50例,其中急性心肌梗死(AMI)组20例,不稳定型心绞痛(UA)组15例,稳定型心绞痛(SA)组15例;健康查体者15例作为正常对照组。采用流式细胞分析法,检测各组外周血Th17、Th17/Th1细胞占CD4+T细胞比例。采用酶联免疫吸附法检测患者血浆IL-17和IL-22的浓度。结果AMI组和UA组外周血Th17细胞比例[(2.98%±1.01%)和(2.63%±0.61%)]、Th17/Th1细胞比例[(0.71%±0.35%)和(0.66%±0.31%)]均明显高于SA组及正常对照组(均P<0.05)。AMI组血浆IL-17(24.41±7.95 ng/L)和IL-22(34.18±7.04 ng/L)浓度均显著高于SA组和正常对照组(均P<0.05);UA组血浆IL-17浓度(22.86±8.62ng/L)显著高于正常对照组,血浆IL-22浓度(28.98±4.35 ng/L)显著低于AMI组而高于正常对照组。ACS组IL-22浓度与IL-17浓度呈显著正相关(r=0.422,P<0.01),且ACS组Th17细胞与IL-17(r=0.722,P<0.01)、IL-22(r=0.400,P<0.01)浓度均呈显著正相关。结论 Th17细胞可能参与动脉粥样斑块不稳定和ACS的发病。Th17细胞及其相关炎性因子IL-17、IL-22可作为诊断急性心血管事件发生的辅助检测指标。
Aim To investigate the alteration and clinical significance of T-helper17 cells (Thl7), interleukin (IL)-17 and IL-22 in patients with aeute coronary syndrome (ACS). Method 50 patients were divided into three groups : acute myocardial infarction ( AMI, n = 20 ) , unstable angina ( UA, n = 15 ) , stable angina ( SA, n = 15 ) ; 15 healthy subjects from physical examination were used as normal control group. The peripheral blood of Thl7 and Thl7/ Thl cells accounted for the proportion of CD4 + T cells were detected by flow eytometry ( FCM ) ; the concentration of plasma IL-17 and IL-22 were detected by enzyme-linked immunosorbent assay (ELISA). Results The proportion of Thl7 cells in AMI group and UA group ( (2.98%±1.01% ) and (2.63%±0. 61% ) ), also the proportion of Thl7/Thl cells in AMI group and UA group ( (0.71%±0. 35% ) and (0. 66%±0. 31% ) ), were both significantly higher than SA group and normal control group (both P 〈 0. 05 ) ; IL-17 and IL-22 concentration in AMI group (IL-17 : 24.41 ± 7.95 ng/L; IL- 22 : 34. 18 ± 7.04 ng/L) were significantly higher than SA group and normal control group ( both P 〈 0. 05 ). IL-17 concentration in UA group (22. 86 ± 8.62 ng/L) was significantly higher than normal control group; IL-22 eoncentration in UA group (28.98 ±4. 35 ng/L) was significantly lower than AMI group, significantly higher than normal control group. In ACS group, there was a significant positive correlation between IL-17 concentration and IL-22 concentration ( r = 0.422, P 〈 0. 01 ), also there was a significant positive correlation between the proportion of Thl7 cells and IL-17 concentration( r =0. 722, P 〈 0. 01 ), IL-22 concentration( r = 0. 400, P 〈 0. 01 ). Conclusions Thl7 cells may be involved in the in- cidence of the atheroselerotie plaque instability and ACS. IL-17 and IL-22 can be used as a secondary detection of ACS diagnosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第4期336-340,共5页
Chinese Journal of Arteriosclerosis