甜叶菊叶片高频再生体系的建立

Establishment of High Frequency Regeneration System of Stevia rebaudiana Bertoni Leaf

以"守田3号"甜叶菊叶片为外植体,研究了消毒时间、外源激素等因素对外植体成活率、不定芽的诱导、不定芽继代培养和生根的影响。结果表明:用0.1%的升汞处理3min为最佳消毒方法,最佳的不定芽诱导培养基为MS+2.0mg/L 6-BA+1.0mg/L IAA+300mg/L水解酪蛋白,平均诱导率为80%;最佳不定芽继代培养基为MS+0.5mg/L 6-BA+0.05mg/L NAA+0.2mg/L GA3+4.0mg/L KT;最佳生根培养基为1/2MS+0.2mg/L IAA,生根率为100%,所获得的再生苗生长健壮且移栽后成活率高,最终建立了甜叶菊最佳的再生体系。

Taking young leaves of Stevia rebaudiana Bertoni as explants,the effects of disinfection time and exogenous hormones on the survival rate,induction of in vitro shoot,growth and root formation were studied. The results showed that the optimum disinfection method was treated with 0. 1% HgCl2 for 3 min; the optimum adventitious buds induction medium from leaves was MS＋2. 0 rng/L 6-BA＋1. 0 mg/L IAA＋300 mg/L CH （Casein Acid Hydrolysate） and the average induction rate was 80% ;the optimum shoot growth medium was MS＋0. 5 mg/L 6-BA＋0. 05 mg/L NAA＋0. 2 mg/L GA3＋4. 0 mg/L KT; the optimum rooting medium was 1/2MS＋0. 2 mg/L IAA with rooting rate 100%, The tissue cultured plants obtained grew well and had high survival rate after transplanting, the optimum regeneration system of Stevia rebaudiana Bertoni was found finally.