Caveolin-1、P63及CK34βE12在59例前列腺癌中的表达及意义

Expression of Caveolin-1,P63 and CK34βE12 in 59 cases of prostate cancer and its clinical significance

目的探讨窖蛋白-1(Caveolin-1)、P63及CK34βE12蛋白在前列腺病变中表达及其临床意义。方法应用免疫组织化学(SP)法检测59例前列腺癌(PCa)、15例高级别前列腺上皮内瘤变(HGPIN)、11例低级别前列腺上皮内瘤变(LGPIN)、7例非典型性腺瘤样增生(AAH)、10例基底细胞增生(BCH)、69例前列腺良性增生(BPH)标本中的Caveolin-l、P63及CK34βE12的表达情况。结果 Caveolin-1、P63、CK34βE12抗原在PCa和BPH标本中的表达差异均有统计学意义(P<0.05)。PCa、HGPIN、LGPIN、AAH、BCH、BPH中Caveolin-1阳性表达率为69.49%(41/59)、40.00%(6/15)、45.45%(5/11)、28.57%(2/7)、30.00%(3/10)、8.70%(6/69),P63阳性表达率为8.47%(5/59)、86.67%(13/15)、90.91%(10/11)、85.71%(6/7)、90.00%(9/10)、97.10%(67/69),CK34βE12为8.47%(5/59)、93.33%(14/15)、90.91%(10/11)、100.00%(7/7)、90.00%(9/10)、95.65%(66/69)。Caveolin-1表达强弱与PCa的Gleason评分、临床分期相关(P<0.05)。结论 Caveolin-1与PCa的发生、发展、侵袭、转移有关,联合检测Caveolin-1、P63及CK34βE12的表达将对PCa早期诊断的准确性、判断病情以及预后有一定临床指导意义。

Objective To investigate the expression of Caveolin-1,P63 and CK34βE12 protein in prostate lesions and its clinical significance.Methods The immunohistochemical staining was adopted to detect the expression of caveolin-1,P63 and CK34βE12 protein in the specimens from 59 cases of prostate cancer（PCa）,15 cases of high grade prostatic intraepithelial neoplasia（HGPIN）,11 cases of low grade prostatic intraepithelial neoplasia（LGPIN）,7 cases of atypical adenomatous hyperplasia（AAH）,10 cases of basal cell hyperplasia（BCH） and 69 cases of benign prostate hyperplasia（BPH）.Results The expressions of caveolin-1,P63,CK34βE12 antigen in PCa and BPH specimens were statistically different（P0.05）.The positive expression rates of caveolin-1 in PCa,HGPIN,LGPIN,AAH,BCH and BPH were 69.49%（41/59）,40.00%（6/15）,45.45%（5/11）,28.57%（2/7）,30.00%（3/10）,8.70%（6/69） respectively,the positive expression rates of P63 were 8.47%（5/59）,86.67%（13/15）,90.91%（10/11）,85.71%（6/7）,90.00%（9/10）,97.10%（67/69） respectively,the positive expression rates of CK34βE12 were 8.47%（5/59）,93.33%（14/15）,90.91%（10/11）,100.00%（7/7）,90.00%（9/10）,95.65%（66/69） respectively.The expression of caveolin-1 and its strength were related to the Gleason score of PCa and clinical stage（P0.05）.Conclusion The expression of caveolin-1 may be related to the development,progress,invasion and metastasis in PCa.Combined detection of the expression of caveolin-1,P63,and CK34βE12 has some guidance significance to the accuracy of early diagnosis,the disease condition judgment and prognosis of PCa.