纤毛虫施氏腹柱虫细胞纤毛器微管的激光共聚焦显微观察

Observations of cell ciliature microtubules of Gastrostyla steinii (Protozoa,Ciliophora) using laser scanning confocal microscopy

应用荧光紫杉醇荧光标记结合激光共聚焦显微术显示,土壤纤毛虫施氏腹柱虫(Gastrostyla steinii)细胞纤毛器微管胞器中,口围带和波动膜后端汇合处含口底托架及口后微管束;额腹横棘毛基部含前纵微管束、后纵微管束和横微管束;左、右缘棘毛基部含前纵微管束、后纵微管束、横微管束和基部微管芽。与目前了解的纤毛虫同类微管结构相比较,施氏腹柱虫显示出其适应于土壤环境而与水生纤毛虫有所区别的一些基本特征,其棘毛基部微管的组成及定位具有该种土壤纤毛虫细胞的特异性。形态发生中,老口围带全部保留,老的或先存的微管结构对新结构的发生和形成具有定位和物质贡献的作用,且老结构在新结构分化和成熟期间也经历着行使相应的生理功能及逐渐退化的过程。

The ciliature microtubular organelles in the soil ciliate,Gastrostyla steinii were visualized by fluorescent labeling of FLUTAX and observed by laser scanning confocal microscopy.The confluence of adoral zone of membranelles（AZM） and undulating membranes（UM） contained the oral-end bracket and post-oral microtubules.The anterior longitudinal microtubules（ALM）,posterior longitudinal microtubules（PLM） and transverse microtubules（TM） were located in the basal part of frontal-ventral-transverse cirri（FVTC）.ALM,PLM,TM and small sub-ectoplasmic rootlets（SSR） were situated in the basal parts of left marginal cirri（LMC） and right marginal cirri（RMC）.Compared with similar microtubule structures of known ciliates,Gastrostyla steinii showed some basic adaptive characteristics to the soil environment and these features were different from those of aquatic ciliates.The composition and location of cirrus base associated microtubules might be of species specificity.During morphogenesis,the ＂old＂ AZM were retained intact as the proter AZM,and the old or pre-existed microtubular structures might play an important role in the location and substance supply of new structures when these structures begin to occur and develop.Furthermore,during the period of differentiation and maturation of the new structures,the old microtubular structures seemed to play their corresponding physiological function when they gradually disintegrated.