基于酶解后乙醇萃取香草兰净油的GC-MS分析

Analysis by GC-MS for Vanilla Net Oil from Ethanol Extraction After Enzyme Hydrolysis

对不同时间酶解后乙醇萃取的香草兰净油进行成分比较,探讨酶解对萃取样品成分的影响。利用酶解不同时间作为预处理,采用乙醇萃取香草兰净油,气相色谱-质谱(GC/MS)联用技术进行分析,分别对5个酶解时间下得到的净油分析。结果表明:酶解6 h作为预处理乙醇萃取后分离出化合物种类最多,峰面积总量最高,其中香兰素、正十六酸、亚油酸、1,2-苯二甲酸单(2-乙己基)酯、16-甲氧基-14-氧化十六碳-15烯酸、甲酯等14种化合物为共有组分,其相对含量占总含量的50%以上。酶解不同时间预处理乙醇萃取的香草兰净油的主要化学成分较为相似,有醛类、酯类、酸、醇、杂环化合物等,但在含量上有较大的差别。

Chemical compositions are compared of vanilla net oil from ethanol extraction after different time enzyme hydrolysis to explore the influence of enzyme hydrolysis on the chemical composition. Use different time enzyme hydrolysis as pretreatment,using ethanol extraction vanilla net oil ,gas eheromatography/mass spectrometry（GC/MS） technologies to analyze the net oil got under five time conditions, respectively,. The results show that： as preprocessing, after 6 h enzyme hydrolysis for , ethanol extraction, the separated out compounds were with most types and the to- tal peak area was Highest. Among them vanillin, n - Hexadecanoic acid, linoleic acid, 1,2 - Benzenedic, mono （ 2 - ethylhexyl） ester, 16 - Methoxy - 14 - oxohexadec - 15 - enoic acid, methyl ester etc. 14 compounds are mutual components. The relative content for the total content was more than 50%. In the different time enzyme hydrolysis, as pretreatment, major chemical composition of ethanol extraction vanilla net oil are more similar, such as aldehyde, esters, acid, alcohol, heterocyclic and other compounds, but in the content they have bigger difference.