725杨组培繁殖技术研究

Research on tissue culture and propagation of Populus deltoides cl.‘725’

以美洲黑杨725杨树(Populus deltoides cl.‘725’)叶片为材料,对其再生体系的建立及其分化和生根苗对潮霉素B的浓度筛选进行了研究。结果表明,叶片的最佳消毒体系为75%的酒精消毒时间8 s,0.1%的升汞消毒3 min,最佳诱导愈伤的培养基是MS+6-BA 0.2 mg·L-1+2,4-D 1.5 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;最佳诱导分化培养基为MS+6-BA1.0 mg·L-1+NAA 0.3 mg·L-1+KT 0.3 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;适宜的继代增殖培养基为MS+6-BA 0.3 mg·L-1+NAA 0.05 mg·L-1+蔗糖25 g·L-1+琼脂6 g·L-1;最佳生根培养基为1/2MS+NAA 0.01 mg·L-1+IBA 0.7 mg·L-1+蔗糖20 g·L-1+琼脂6 g·L-1;对725杨叶片分化和不定芽生根进行了潮霉素B的敏感性试验,确定叶片分化的临界浓度为2.5 mg·L-1,生根的临界浓度为1.5 mg·L-1。

In this study,the regeneration system was established from leave explants of Populus deltoides cl.‘725’.Meanwhile,the buds and rooting seedlings of Populus deltoides cl.‘725’ were screened using hygromycin B.The results indicated that the best disinfection time of leaves was using 75% of the alcohol for 8 s and 0.1% HgCl2 for 3 min;The best suitable medium of deltoides cl.‘725’ leaves for callus induction was MS＋6-BA 0.2 mg·L^-1＋2,4-D 1.5 mg·L^-1＋sucrose 25 g·L^-1＋agar 6 g·L^-1;appropriate induction differentiation medium was MS＋6-BA 1.0 mg·L^-1＋NAA 0.3 mg·L^-1＋KT 0.3 mg·L^-1＋ sucrose 25 g·L^-1＋agar 6 g·L^-1;the best multiplication media was MS＋6-BA 0.3 mg·L^-1＋NAA 0.05 mg·L^-1＋sucrose 25 g·L^-1＋agar 6 g·L^-1;the best medium for rooting was 1/2 MS＋ NAA 0.01 mg·L^-1＋ IBA 0.7 mg·L^-1＋ sucrose 20 g·L^-1＋agar 6 g·L^-1;the critical hygromycin B sensitive concentrations for inducing shoots and roots of the poplar were 2.5 mg·L^-1and 1.5 mg·L^-1,respectively.