摘要
目的通过观察高频电刺激帕金森(PD)模型大鼠脚桥核(PPN)对苍白球内侧部(GPi)氨基酸类递质及神经元放电的影响,探讨电刺激PPN治疗PD的机制。方法将70只SD雄性大鼠随机分为对照组30只,PD模型组40只。大脑右侧黑质致密部两点注射6-羟基多巴胺建立PD模型,应用细胞外单位记录观察高频电刺激PPN对GPi神经元放电的影响。脑内微透析收集损毁侧GPi脑脊液,结合高效液相色谱检测氨基酸类递质的变化。结果高频电刺激PPN,对照组及PD模型组大鼠GPi神经元的反应均以兴奋性为主,且平均放电频率均较刺激前明显增高(P﹤0.01);高频电刺激PPN,GPi内谷氨酸水平较刺激前明显减少(P﹤0.01),γ-氨基丁酸水平较刺激前无明显变化(P﹥0.05)。结论高频电刺激PPN可增强GPi神经元的电活动,降低GPi内谷氨酸的水平,其兴奋GPi神经元的效应可能是通过PPN-GPi直接通路或其他间接通路实现的。
Objective To study the effect of high frequency stimulation （HFS） in pedunculopontine nucleus （PPN） on the neuronal activities of globus pallidus internus （Gpi） in Parkinson＇ s disease （PD） model rats, and the mechanisms thereof. Methods Seventy male Sprague-Dawley rats were divided into two groups, control group （n=30） and PD model group （n= 40）. PD rat model was established by the injection of 6-OHDA into substantia nigra pars compacta （SNc） on the right side of the brain with stereotactic technique. Electrophysiological recordings were made in anaesthetized rats to investigate the ef fects of HFS-PPN on the firing rate of the GPi neurons. Brain microdialysis combined with high-performance liquid chromatography was applied to detect glutamate （Glu） and Y-aminobutyric acid （GABA） levels in GPi. Results HFS-PPN caused an excitatory reaction of the majority of neurons recorded in the GPi in PD model group and control group. The mean firing rate of GPi excited neurons was significantly increased （P 〈 0.01）. The levels of Glu were reduced under HFS-PPN and the levels of GABA were not affected （P 〉 0.05）.Conclusion HFS-PPN heightened the electrical activity of GPi neurons and reduced the level of Glu. These excitatory effects were probably realized by PPN-GPi direct path or other indirect path.
出处
《天津医药》
CAS
北大核心
2013年第7期682-685,共4页
Tianjin Medical Journal
基金
辽宁省教育厅科研项目(项目编号:L2010252)
