湖北麦冬有效部位总皂苷的提取纯化工艺研究

Study on Extraction and Purification Technology of Hubei Ophiopogon Saponins

目的:研究湖北麦冬有效部位总皂苷分离纯化的工艺。方法:采用正交试验优选麦冬总皂苷提取物的最佳提取工艺,采用大孔树脂分离纯化总皂苷,并进行验证试验;采用紫外分光光度法进行含量测定。结果:最佳提取工艺为:10倍量70%乙醇加热回流提取3次,每次2 h;选用D101树脂进行纯化,50%～70%乙醇对总皂苷的洗脱效果最好,可以选择70%的乙醇作为洗脱剂;工艺验证与试验结果具有很高的一致性。结论:该工艺简单,稳定可行,提取率高,可以为湖北麦冬中药新药有效部位研究和生产提供参考。

Objective ：To explore the extraction and purification technology of total saponins from the effeetive parts of Liriope spi-cata . Methods：Orthogonal design was used. Macroporous resin was selected to separate and purify total saponin fi＇om the effective parts of Liriope spicata. The process validation was conducted. The total saponins was determined by Ultraviolet Spectrophotometry. Results ： The optimal extraction conditions were as follows： 10 times the amount of ethanol（70% ） for each occasion and hot reflux（3 × 2 h）. To-tal saponins was purified by D101 macroporous resin. The concentration of eluting ethanol was 50% - 70%. Ethanol（70% ） was selected as the best eluent. The result of process validation was consistent with the study. Conclusion ：The process is simple and stable enough to significantly improve the extraction rate of the effective parts. The study can provide reference for the research and production of effec-tive parts of traditional Chinese medicine such as Liriope spicata.