纳米板转导HPV16 E7siRNA对宫颈肿瘤组织增殖和凋亡的影响

Effects of Nanopatch Delivering HPV16 E7siRNA on Proliferation and Apoptosis of Cervial Cancer

目的:探讨经纳米板转导HPV16 E7siRNA对宫颈癌组织增殖和凋亡的影响。方法:建立裸鼠宫颈癌移植瘤模型,纳米板将HPV16 E7siRNA转导入肿瘤组织细胞,转导后2 d用TUNEL法检测实验组和对照组的凋亡情况,免疫组化法检测实验组和对照组Ki-67的表达情况。结果:经纳米板转导HPV16 E7siRNA后实验组细胞凋亡显著增加,Ki-67表达明显降低,与对照组相比凋亡指数、增殖指数差异均具有显著性(P<0.05)。结论:纳米板转导的HPV16 E7siRNA对宫颈癌肿瘤组织增殖具有明显的抑制作用。

Objective： To evaluate the effects of nanopatch delivering HPV16 E7siRNA on proliferation and apoptosis of cervical cancer.Methods： Set up cervical cancer nude models,with nanopatch deliveried HPV16 E7siRNA into the transplanted tumor of nude mice models;apoptosis and the expression of Ki-67 were detected by TUNEL and immunohistochemistry staining at 2 days after treatment.Results： After treatment there were many TUNEL positive cells in experimental group,the expression of Ki-67 decreased significantly,the difference of apoptosis index and proliferation index between experimental group and control group was significant（P 0.05）.Conclusions： Using nanopatch to deliver siRNA to cervical cancer can induce apoptosis and inhibiting of the proliferation significantly.