趋化因子mRNA在实验性变态反应性神经炎中表达的研究

C-C Chemokine mRNA expression in murin experimental allergic neuritis

目的:实验性变态反应性神经炎(EAN)是一类T细胞介导的周围神经系统的自身免疫病,可用牛坐骨神经加完全氟氏佐剂诱导而成。本文研究趋化因子mRNA在实验性变态反应性神经炎(EAN)中的表达并探索其可能的作用。方法:用兔坐骨神经匀浆免疫Wistar大鼠,诱导格林巴利综合症(GBS)的动物模型EAN;采用地高辛标记的寡核苷酸探针检测EAN病变神经组织浸润细胞上趋化因子单核细胞趋化蛋白-1(MCP-1)及巨噬细胞炎性蛋白-1β(MIP-1β)mRNA表达情况。结果:MCP-1mRNA在临床症状出现前1-2天(14天)水平最高,随后逐渐下降;MIP-1 βmRA在临床症状出现前1-2天水平开始升高,在临床症状达到高峰时(21天)最高,进入恢复期后降至基础水平。结论:趋化因子在EAN的炎性细胞迁移及浸润进入神经细胞过程中起到重要作用。

Objective: Experimental autoimmune neuricis (EAN) is a T-cell-mediated autoimmune disease of the peripheral nervous system (PNS) that can be actively induced in Lewis rats by imunization with bovine PNS myelin and Freund's complete adjuvant. In the present study, the dynamics of the expression of C-C chemokines mRNA of macrophage inflanr matory protein-1 β(MIP-1β) and monocyte chemotactic protein-1(MCP-1) were determined in the sciatic nerves of EAN. Methods: The mRNA of MIP-1β and MCP-1 were identified in infiltrating cells in sciatic nerve sections over the course of EAN by in situ hybridization (ISH) using digoxingenin labeled riboprobes. Results: The maximum of mRNA encoding MIP-1β positive cells in the scitic nerves was seen en day 21 post immunization (p. i. ) correlating with the development of severe clinical signs. Peak numbers of MCP-1 mRNA positive cellswere induced in the infiltrating cells in sciatic nerves l-2days before clinical signs were apparent of EAN, favoring a role for these chemokines in disease development. Conclusion: These results define a subset of chemokines that may play an important role in the inflammatory process during muring EAN and have relevance for future studies on pathogenesis and treatment of GBS in humans.