羟基喜树碱联合放射对人肺腺癌细胞协同杀伤作用的实验研究

The effect of HCPT combined with radiation on cell proliferation of human lung adenocarcinoma cell line (D_6) in vitro

目的 研究羟基喜树碱联合放射对人肺腺癌细胞的协同杀伤作用。方法 应用四唑盐比色试验 (MTT)检测细胞抑制百分比 ,DNA琼脂糖电泳检测DNA“Ladder”及流式细胞术检测细胞周期及凋亡峰的比例。结果 羟基喜树碱对该细胞的抑制呈剂量及时间依赖关系 ,且明显阻滞细胞于S期 ,低浓度的羟基喜树碱 (1μg/ml)作用 48小时 ,细胞开始出现凋亡小峰 ,高浓度的羟基喜树碱 (5 0 μg/ml)作用 2 4小时就出现明显的凋亡小峰。单纯放射 (6Gy)后 48小时细胞出现DNA“Ladder”条带 ,单纯羟基喜树碱 (1μg/ml)作用后 2 4～ 72小时 ,细胞出现DNA“Ladder”条带及凋亡小峰 ,细胞经羟基喜树碱联合放射作用后的 0～ 72小时均出现DNA“Ladder”条带及凋亡小峰。

Objective To study the adjuvant effects of HCPT and radiation on cell proliferation of human lung adenocarcinoma cell line (D 6) in vitro. Methods Suppression rate of cell growth, apoptosis and cell cycle of human pulmonary adenocarcinoma cell line were detected by MTT assay, DNA agrose electrophoresis and flow cytometry respectively. Results The suppression effects of HCPT on cell proliferation were dose and time dependent. The cells were significantly blocked at S stage by HCPT. Apoptosis peak appeared at 24 and 48 hours after treatment with high concentration (50?μg/ml) and low concentration of HCPT (1?μg/ml) respectively. DNA “Ladder” started to appear at 48 hours by radiation alone, but DNA “Ladder” and apoptosis peak both appeared at 24～72 hours and 0～72 hours after treatment with HCPT alone and HCPT combined with radiation respectively. Conclusion HCPT combined with radiation may show the synergistic killing effect on human lung adenocarcinoma cell line (D 6) in vitro.