PKC基因在人肺癌中的转录表达

The transcript expression of PKC-β_1 gene in human lung cancer

目的 探讨PKC β1基因转录表达与肺癌临床病理生理特征的关系。方法 应用Northern印迹杂交方法检测 5 0例肺癌组织、癌旁肺组织、远癌肺组织和 30例肺良性病变肺组织PKC β1mRNA表达水平。结果 (1)肺癌组织中PKC β1mRNA表达水平显著高于癌旁肺组织 ,远癌肺组织和肺良性病变肺组织 (P <0 .0 1) ;(2 )低分化肺癌中PKC β1表达水平显著高于中 高分化肺癌 (P <0 .0 5 ) ;(3)Ⅲ期肺癌PKC β1表达水平显著高于Ⅰ、Ⅱ期肺癌 (P <0 .0 1) ;(4)伴有淋巴结转移肺癌PKC β1表达水平显著高于不伴淋巴结转移肺癌(P <0 .0 1)。结论 肺癌组织中存在PKC β1mRNA过度表达 ,它可能参与肺癌细胞分化以及肿瘤进展和转移过程的调控。

Objective To determine the relationship between the transcript expression of PKC β 1 gene and pathophysilogical characteristics of lung cancer. Methods The expression of PKC β 1 mRNA was detected in 50 human lung cancer tissues, para cancerous tissues, non cancerous tissues and 30 benign pulmonary lession tissues by Northern blot. Results (1) The expression level of PKC β 1 mRNA in lung cancer tissues was significantly higher than that in para cancerous tissues, non cancerous tissues and benign pulmonary lession tissues (P<0.01). (2) The expression level of PKC β 1 in poor differentiated cancer was significantly higher than that in moderate well differentiated cancer (P<0.05).(3) The expression of PKC β 1 mRNA in stage Ⅲ cancer was remarkably higher than that in stage Ⅰ and stage Ⅱ disease (P<0.01). (4) The expression of PKC β 1 in cancer with lymph node metastasis was significantly higher than that in the cancer without lymph node involvement (P<0.01). Conclusion There is an overexpression of PKC β 1 mRNA in lung cancer tissues, and it may play an important role in the advancement, cancer cell differentiation and metastasis of lung cancer.