离子对高效液相色谱法同时测定氧化型染发剂中5种染料

Simultaneous Determination of Five Dyes in Hair Dying Formulations by Ion-Pair HPLC

采用离子对高效液相色谱法同时测定氧化型染发剂中5种染料成分,选择Phenomenex kinetex C18色谱柱(2.6μm,4.6 mm×150 mm),以10 mmol/L辛烷磺酸钠水溶液(pH值2.5)+甲醇(体积比60∶40)为流动相,采用DAD检测器,检测波长280 nm,以保留时间和紫外光谱特征定性,外标法定量。5种染料在20.0 mg/L～100 mg/L范围内线性关系良好,最低检出质量分数氢醌为0.02%,间苯二酚为0.03%,邻氨基苯酚为0.02%,邻苯二胺为0.04%,4-氨基-2-羟基甲苯为0.03%。低浓度基质加标平均回收率为82.5%～106%,RSD为1.4%～6.1%,中浓度基质加标平均回收率为82.0%～105%,RSD为0.4%～2.2%,高浓度基质加标平均回收率为78.5%～105%,RSD为1.7%～4.5%。

An ion-pair high performance liquid chromatographic method was established to simultaneous determination five dyes in oxidized hair dyes. The chromatographic conditions were as follows： Phenomenex kinetex Cls column （ 2.6 μm, 4.6 mm × 150 mm ） was selected, with 10 mmol/L Oetanesulfonic acid sodium solution （ pH 2.5 ）-methanol （ 60：40, V/V） as the mobile phase, a diode array detector was used, detection wave- length at 280 nm, qualitative analysis by retention time and UV spectra characteristics, quantitative analysis by external standard method. Results showed good linearity for the 5 compounds ranging from 20. 0 mg/L to 100 mg/L, lowest detective limits for Quinhydrone is 0.02% , Resorcinol is 0.03% , o-aminophenol is 0.02% , o-phenylenediamine is 0.04%, 4-amino-2-hydroxytoluene is 0.03%. Average spiked recoveries of matrices at lower level were between 82.5% and 106% ,with RSD between 1.4% and 6.1%. Average spiked recoveries of matrices at middle level were between 82.0% and 105% ,with RSD between 0.4% and 2.2%. Average spiked recoveries of matrices at high level were between 78.5% and 105% ,with RSD between 1.7% and 4.5%.