超声对软骨细胞凋亡、caspase-8和caspase-3表达的影响

Ultrasound effects on chondrocyte apoptosis and the expressions of caspase-8 and caspase-3

背景:超声治疗能够缓解疼痛,改善膝骨关节炎患者的运动功能,但目前关于超声治疗的文献缺乏一致性。目的:进一步验证超声治疗的膝骨关节炎有效性。方法:24只兔子随机分为3组,正常组不干预;模型组兔行前交叉韧带离断诱导膝骨关节炎,不接受任何治疗;超声组兔建模后接受超声波治疗10 min/次,1次/d,0.3 W/cm2,1 MHz,共治疗10次。采用苏木精-伊红染色进行兔关节软骨组织学观察,运用免疫印迹和RT-PCR技术检测兔软骨中的caspases3和caspases8的表达,TUNEL技术检测兔膝骨关节软骨细胞凋亡率。结果与结论:正常兔软骨组织软骨细胞呈柱状整齐的排列,模型中软骨层变薄,软骨细胞排列无序而少。超声治疗后软骨细胞重新排列,趋于整齐,细胞数增加。模型组为膝骨关节炎模型;与正常组比较,模型组和超声组改良Mankin评分较高,模型组和超声组软骨细胞凋亡指数更高,模型组高于超声组。与正常组比较模型组和超声组caspase-3和caspase-8表达较高,超声治疗后两项指标表达下降。结果表明,超声可以改善软骨组织结构、降低caspase-3、caspase-8的表达,降低软骨细胞凋亡。提示超声治疗膝骨关节炎是有效的。

BACKGROUND： Ultrasound therapy can relieve pain and improve the movement function in patients with knee osteoarthritis, but there lacks of consistency in the literatures of ultrasound therapy. OBJECTIVE： To further identify the effectiveness of ultrasound therapy in the treatment of knee osteoarthritis. METHODS： Twenty-four rabbits were randomly divided into three groups： normal group, model group and ultrasound group. The rabbit in the normal group received no intervention; rabbits in the model group received anterior cruciate ligament transaction to establish the knee osteoarthritis model without any treatment; the rabbit in the ultrasound group received ultrasound therapy after modeling for 10 minutes once time, once per day, 0.3 W/cm2, 1 MHz and treated for 10 times. Hematoxylin-eosin staining was conducted for histological observation of rabbit articular cartilage; western blot and reverse transcription PCR assessment were used to assess the expressions of caspases-3 and caspases-8 in rabbit articular cartilage, while Terminal deoxynucleotidyl transferase dUTP nick end labeling was used to assess the ratio of chondrocytes apoptosis of rabbit knee articular cartilage. RESULTS AND CONCLUSION： The normal rabbit cartilage tissues and chondrocytes were neatly arranged in column; the middle cartilage layer of the model was thin; the chondrocytes were arranged disorderly and became less. After ultrasound therapy, the chondrocytes were rearranged neatly, and the number was increased. Compared with the normal group, the Mankin scores in the model group and ultrasound group were higher; the apoptotic rate of chondrocytes was higher in the model group and ultrasound group than in the normal group,