磁悬浮离心式左心室辅助装置溶血实验研究

Hemolysis Test on a Magnetic Suspension Centrifugal Ventricular Assist Device

通过体外以及在体溶血实验,探讨磁悬浮驱动离心式心室辅助装置血液相容性能.将左心室辅助装置置于充满新鲜羊血的模拟循环通路中,在输出流量为5 L/min、后负荷压力为100 mmHg的条件下,检测血泵体外溶血性能.在8只健康绵羊体内植入血泵进行在体辅助实验,通过定期抽血检验血浆游离血红蛋白值,测定其在体溶血性能.结果表明:可植入磁悬浮驱动离心式左心室辅助装置的体外标准溶血指数(normal index of hemolysis,NIH)为(0.075±0.017)mg/L.在体实验中,2例存活超过20 d,循环辅助后实验羊血浆游离血红蛋白(free hemoglobin,FHB)呈现上升趋势,2例实验FHB最大值分别达0.846和1.09 g/L,之后逐渐下降到术前水平.本实验血泵同其他国产血泵比较,具有较好的体外和在体血液相容性,具备好的长期动物在体辅助实验基础.

To explore the hemolytic characteristics of a self-made implantable magnetic suspension centrifugal ventricular assist device in vitro and in vivo. The blood pump of the ventricular assist device was connected in a mock circulatory loop, filled with fresh sheep blood, and the pump generated 5 L/min flow against a head pressure of 100 mmHg. The vitro hemolytic properties were evaluated by determining the level of plasma free hemoglobin （FHB） at defined intervals. Blood pump was implanted in two healthy sheep in vivo test. After pumping, FHB and hemoglobin （HB） were detected continuously during the experiments. The normal index of hemolysis （NIH） in vitro was （0. 075 ±0. 017） mg/L. Vivo experiment results showed that the levels of FHB gradually increased, and the maximal mean values were 0. 846 and 1.09 g/L, respectively. Compared with others domestic LVADs, the vitro and vivo hemolyticproperties of this blood pump are satisfactory. Therefore, long-term circulation assist experiment of the new blood pump can be carried out to evaluate its hemodynamic and end-organ effect in the future.