EDC/NHS交联胶原基牙周组织引导再生膜材料的研究

Cross-linked Collagen Guided Tissue Regeneration Membrane with EDC /NHS

采用胶原材料制备了一种密实-疏松双层结构的牙周引导再生膜材料.为改善再生膜材料的物理化学性能,采用1-乙基-3-(3-二甲基氨丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)为交联剂对膜材料进行交联,考察了不同交联剂质量浓度对膜材料物理化学性能的影响,并通过差示扫描量热分析仪(DSC)、扫描电镜(SEM)、吸水率测试、膨胀动力学分析、抗酶解性能分析等手段对膜材料交联前后的结构与性能进行了研究.结果表明,以EDC/NHS为交联剂,在pH为5.5、EDC质量浓度为5 g/L、交联时间为24 h的条件下,引导再生胶原膜材料的综合性能达到最佳,进一步提高交联剂的浓度,材料物理化学性能的变化并不明显.采用EDC/NHS交联后,可显著改善再生膜材料的物理化学性能.交联后膜材料的变性温度和抗酶解性能显著提高,并且维持了密实-疏松的双层结构.

A collagen GTR membrane was made with a bi-layer structure, one of whose face is loose while the other is tight. The tight face can effectively prevent the cells from growing into the defective tis- sue zone while the loose face is rough, which is helpful to cells growth and blood clot. In order to im- prove the physicochemical properties of the membranes, EDC/NHS was used as a cross-linking agent for the membranes. And the effects of cross-linking agent dosage on the physicochemical properties of mem- branes were investigated. The measurements of DSC, SEM were used to test the structure and properties of the membranes. The properties of water absorption, collagen resistance were also tested. According to the study, 0.5 mol/L EDC/NHS, cross-linking 24 h in the pH of 5.5 are the best processing conditions. Increasing further the concentration of EDC can not improve the physicochemical properties of the mem- branes. The research shows that the physicochemical properties of collagen membranes are improved sig-nificantly after being cross-linked. The denaturation temperature, anti-collagen activities are both in- creased, and the hi-layer structure is kept well.