摘要
[Objective] This study was aimed to find a new method for the classification of common aroma components in tobacco leaves. [Method] Sixty-four common aroma components in tobacco leaves were classified by cluster analysis based on their relative molecular weight. The contents and distribution of aroma components in another 71 C3F and 64 B2F tobacco leaf samples were analyzed by using the new method. [Result] The 64 common aroma components were divided into three categories trough the cluster analysis based on their molecular weight. CategoryⅠ consisted of 12 aroma components which had high molecular weight(281.308±21.536on average) and high boiling point(371.311±29.904 ℃ on average). Category Ⅱ included 27 components which had low molecular weight(103.722 ± 13.115 on average) and low boiling point(176.132±42.342 ℃ on average). Category Ⅲ included 25components which had middle molecular weight(175.393 ± 24.906 on average) and middle boiling point(250.562±45.431 ℃ on average). The content of high-molecularweight aroma components in middle leaves(547.344±224.391 μg/g) was much higher than that in upper leaves(477.549±182.066 μg/g). The content of low-molecularweight aroma component in middle leaves(17.468±3.459 μg/g) was also significantly higher than that in upper leaves(15.936±3.456 μg/g). The content of middle-molecular-weight aroma component in middle leaves(44.931 ±8.953 μg/g) was extremely significantly higher than that in upper leaves(37.997±6.042 μg/g). [Conclusion] This study proposed a new way to classify the aroma components in flue-cured tobacco leaves using the relative molecular weight as the index, which will provide theoretical reference for developing special tobacco leaves.
[目的]为了探索新的烤烟常见致香物质分类方法。[方法]以烤烟中常见的64种致香物质的相对分子质量为指标,采用聚类分析法对其进行分类、描述,并以中国云南省13个产烟地区的71份C3F和64份B2F烟叶为材料,对比分析了不同相对分子质量大小致香物质含量在其中的分布。[结果]以分子量为指标,64种致香物质可以通过聚类分析被分为3类,第一类是高分子量、高沸点,包括12种致香物质,平均分子量为(281.308±21.536)℃,平均沸点为(371.311±29.904)℃;第二类是低分子量、低沸点,包括27种物质,平均分子量为(103.722±13.115)℃,平均沸点为(176.132±42.342)℃;第三类是中等分子量、中等沸点,包括25种物质,平均分子量为175.393±24.906,平均沸点为(250.562±45.431)℃。大分子量致香物质含量以中部烟叶(547.344±224.391)μg/g显著高于上部烟叶(477.549±182.066)μg/g,小分子量致香物质含量以中部烟叶(17.46g±3.459)μg/g显著高于上部叶(15.936±3.456)μg/g,中等分子量致香物质含量以中部烟叶(44.931±8.953)μg/g极显著高于上部叶(37.997±6.042)μg/g。[结论]该研究提出了一种新的致香物质的分类方法,为特色烟叶的开发提供一定的理论基础。
基金
Supported by the Fund from Hongyun Honghe Tobacco(Group)Co.Ltd.(HYHH2012YL01)~~