抗体应答期间大鼠脑和胸腺中儿茶酚胺含量的变化

CHANGES OF CATECHOLAMINE CONTENT IN THE BRAIN AND THYMUS OF RATS DURING ANTIBODY RESPONSE

目的 :探讨在抗体应答期间 ,脑和淋巴器官中儿茶酚胺 (CAs)含量的动态变化 ,藉以了解免疫状态对中枢和外周CAs神经活动的影响。方法 :用绵羊红细胞 (SRBC)免疫大鼠 ,在免疫后第 2～ 7d应用高效液相色谱 电化学检测法 (HPLC ECD)测定大鼠下丘脑、海马、脑干和胸腺中去甲肾上腺素 (NA)、肾上腺素 (A)、多巴胺 (DA)和高香草酸 (HVA)的含量。结果 :①下丘脑和海马内NA在抗体应答期间升高 ,而胸腺中NA降低 ;②下丘脑、海马和胸腺中A含量在抗体应答期间均增加 ;③脑内DA在抗体应答期间有增加和减少的现象 ,但HVA都增加 ;④脑干中CAs含量在抗体应答期间均无显著变化。结论 :抗体应答可能使下丘脑和海马中CAs神经的活动加强 ,对胸腺CAs的影响可能依递质不同而作用不同 。

Aim: The kinetic changes of catecholamine content in the brain and lymphoid organ of rats during the antibody response were investigated in order to know the effect of immune condition on the activity of catecholaminergic nerve in central and peripheral nervous system. Methods:Rats were immunized with sheep red blood cells (SRBC). During the days 2～7 after immunization, the content of noradrenaline (NA), adrenaline (A), dopamine (DA) and homovanilic acid (HVA) in the hypothalamus, hippocampus, brainstem and thymus of rats was determined by means of the high performance liquid chromatography with electrochemical detection. Results: ①NA content in the hypothalamus and hippocampus significantly increased,but was strikingly decreased in the thymus during the antibody response compared with the saline control. ②The content in the hypothalamus, hippocampus and thymus markedly augmented in the period of antibody response. ③DA content in the hypothalamus significantly increased,but in the hippocampus, DA content markedly descended after immunization. HVA, DA metabolite, dramatically rose in the hypothalamus and hippocampus. ④There were not significant alterations of catecholamine content in the brainstem during the antibody response compared with the saline control. Conclusion: The antibody response may enhance the activity of catecholaminergic nerve in the hypothalamus and hippocampus, and may exert different impact on various catecholaminergic nerve in the thymus, but does not markedly affect the activity of catecholaminergic nerve in the brainstem.