消栓通络胶囊对急性脑缺血再灌注大鼠脑组织TNF-α和IL-1β水平的影响

Effects of XiaoShuangTongLuo Capsule on TNF-α and IL-1β in Cerebral Tissue of Rats with Acute Cerebral Ischemia-reperfusion Injury

目的:观察消栓通络胶囊对急性缺血性中风模型大鼠脑组织肿瘤坏死因子-α(TNF-α)和白介素-1β(IL-1β)水平的影响,以探讨其治疗缺血性中风的作用机制。方法:采用线栓法制备大鼠大脑中动脉缺血再灌注(MCAO)模型,测定大鼠脑组织中TNF-α和IL-1β的水平。结果:与模型组比较,消栓通络胶囊大、中(1.68、0.84 g/kg)剂量组可明显降低MCAO大鼠神经行为学评分(P<0.01),可明显降低MCAO大鼠患侧脑匀浆中TNF-α和IL-1β的水平(P<0.01)。结论:消栓通络胶囊可提高急性脑缺血再灌注大鼠的神经功能活动能力,其治疗缺血性中风的作用机制可能与TNF-α和IL-1β的表达有关。

Objective：To discuss the mechanism of XiaoShuang TongLuo capsule in treating ischemic stroke through observing the effects of the drugs on TNF-αand IL-1βof cerebral tissue of rats with acute ischemic stroke. Method：Rat models with middle cerebral artery occlusion （MCAO） were established, the levels of TNF-αand IL-1βin cerebral tissue were detected. Result： Compared with the model group, high and middle dosage group of Xi-aoShuang TongLuo capsule （1.68 and 0.84g/kg） could obviously decrease the scales of neurological behavior of MA-CO rats remarkably （P〈0.01）, and lower the levels of TNF-αand IL-1βin cerebral homogenate of the affected side in MACO rat （P〈0.01）. Conclusion：XiaoShuang TongLuo capsule could improve the performance and neurological function of the rats with acute cerebral ischemia-reperfusion injury, its mechanism might be related to the expres-sions of TNF-αand IL-1β.