COX-2与MVD在三阴性乳腺癌中的表达及相关性研究

Expression and correlation of COX-2 and MVD in triple-negative breast carcinoma

目的:研究COX-2在三阴性乳腺癌中的表达及意义,探讨COX-2与CD31标记的新生微血管密度(MVD)的相关性。方法:采用免疫组化法检测60例三阴性乳腺癌及518例非三阴性乳腺癌组织石蜡标本中的COX-2和MVD的表达情况,并对三阴性乳腺癌与非三阴性乳腺癌的临床病理特征进行比较。结果:①与非三阴性乳腺癌组相比,三阴性乳腺癌组患者年龄偏轻、绝经前患者比例明显升高;三阴性乳腺癌组织分化程度低,组织学分级为Ⅲ级的比例明显升高;②COX-2在三阴性乳腺癌及非三阴性乳腺癌组织中的阳性率分别为73.3%、58.0%,两者存在差异(P<0.05);③三阴性乳腺癌的MVD为183.73±25.64,非三阴性乳腺癌组织中MVD为168.84±24.07,两者间具有显著差异(P<0.01);④COX-2与MVD在三阴性乳腺癌中的表达具有显著相关性(P<0.01)。结论:三阴性乳腺癌组织分化差,恶性程度高,COX-2表达和MVD在三阴性乳腺癌中显著增高,两者可能共同参与三阴性乳腺癌的发生、发展,检测COX-2和MVD可作为反映三阴性乳腺癌临床及病理学特点的参考指标,同时可考虑将COX-2作为三阴性乳腺癌治疗的新靶点。

Objective：To study the expression and significance of COX-2 in triple-negative breast carcinoma （TNBC） and explore the relevance of COX-2 and neovascularization （MVD） marked by CD31. Methods：The expression of COX-2,MVD in 60 TNBC cases and 518 paraffin wax samples of non-TNBC patients were detected by immuno-histochemical method,and the clinical characteristics of TNBC cases and non-TNBC cases were compared. Results： （1）Compared with non-TNBC patients, TNBC cases were younger and mostly premenopausal patients,the differentiation of TNBC was lower while the percentage of histology graded Ⅲ was significantly increased; （2）The positive rate of COX-2 in 60 cases of TNBC was 73.3%, and that in 518 cases of the non-TNBC was 58.0%. The difference is statistically significant（P 〈 0.05） ;（3）In 60 cases of TNBC,the value of MVD （CD31 marked）was 183.73 ± 25.64,in 518 cases of non-TNBC,MVD was 168.84±24.07 （P 〈 0.01）; @The expression of COX-2 in TNBC was significantly correlated with CD31 （P 〈 0.01）. Conclusion：The high expression of COX-2 and MVD are closely associated with infiltration and metastasis of TNBC, thus the abnormal expression of COX-2 and MVD are likely to participate together in the genesis and progress of TNBC.