摘要
本文用亲合印迹法测定了玉米矮花叶病毒 ( MDMV)与 MDMV HC- Pro,及二者与蚜虫蛋白之间的互作。结果表明 ,MDMV HC- Pro可以与 MDMV结合 ,也可以直接与 MDMV的介体——麦二叉蚜 ( Schizaphis graminum)中分子量约为 56k D的可溶性蛋白结合 ,而 MDMV不能与麦二叉蚜的可溶性蛋白直接结合。无论是 MDMV HC- Pro,还是 MDMV,都不能结合非介体灰飞虱 ( Laodelphaxstriatellus)的可溶性蛋白。酶联板模型的结果与此一致。上述结果说明 ,病毒不能直接与蚜虫口针中的病毒附着位点 ( VAS)结合 ;HC- Pro一端连接病毒 ,一端连接 VAS,在蚜虫传播 MDMV的过程中起桥梁作用。这是关于病毒 HC-
The interactions between maize dwarf mosaic virus (MDMV), HC Pro, and virus attachment site (VAS) were studied by affinity blot (overlay assay of virus protein blot). MDMV HC Pro could bind MDMV in the assay, and vise versa. Only MDMV HC Pro could react with the soluble protein of aphid vectors directly, while MDMV was not. It therefore provided evidence to support the hypothesis that HC Pro acted as a bridge in aphid transmission of virus. Neither CP nor HC Pro was able to interact with any protein of non vectors. The mole cular weight of the aphid protein interacting with MDMV HC Pro was ca. 56 kD. This is the first biochemical evidence of interaction between HC Pro and VAS in aphids.
出处
《植物病理学报》
CAS
CSCD
北大核心
2000年第3期217-221,共5页
Acta Phytopathologica Sinica
基金
攀登计划资助项目! (85 - 31- 0 2 - 0 3)
关键词
玉米矮花叶病毒
辅助成份-蛋白酶
蚜虫传毒
maize dwarf mosaic virus (MDMV)
helper component protease (HC Pro)
virus attachment site (VAS)
bridge hypothesis
