摘要
以耐酸性酒精酵母A3为出发菌株,对其原生质体进行紫外线(UV)与亚硝基胍(NTG)复合诱变,利用三级筛选模式,筛选出一株高产突变菌株UN1-81。该菌株在发酵结束后,酒精度达到11.17%vol,比出发菌株提高了16.47%以上,且经过20次传代培养,酒精产量稳定。
In this experiment, acidproof alcohol yeast strain A3 was used as original strain and its protoplast was treated by UltraViolet（UV）-Nitrosoguanidine （NTG）composite mutation to obtain acid-proof and high ethanol-producing mutants, then a quality strain UN1-81 was finally screened by three-class screening mode from large quantity of flat plates. The mutated strain after fermentation could obtain 11.17% （Vol） alcohol concentration which enhanced 16.47 % to start strain, and the stable genotype with high ethanol yield had been verified through twenty times of transferring of culture.
出处
《酿酒》
CAS
2013年第6期65-68,共4页
Liquor Making
关键词
酒精酵母
耐酸
原生质体
紫外辐照
亚硝基胍
复合诱变
alcohol yeast
acidproof
protoplast
ultraviolet radiation
DES
composite mutation
