风疹IgM抗体荧光免疫层析法的建立

Development of rubella virus IgM antibody immunity chromatography assay using fluorescence lanthanide nanoparticles

目的建立了检测血清中风疹病毒IgM抗体的荧光免疫层析法。方法通过对风疹病毒包膜蛋白E1与荧光颗粒偶联条件、质控线包被条件、检测线包被条件的优化,成功建立了风疹IgM抗体荧光免疫层析法。结果以ELISA为参考,该方法的灵敏性、特异性和符合率分别为77.2%、82.3%、79.3%,其中灵敏性和符合率两项指标均优于胶体金免疫层析法,特异性与胶体金免疫层析法相当。结论本研究建立的检测风疹IgM抗体的荧光免疫层析法,具有简便、快速、灵敏度高的特点,克服了目前风疹病毒IgM检测中存在的操作繁琐、检测时间长、检测费用高的缺点,有潜在的应用价值。

Objective In this study,rubella virus E1 envelope protein was used as detection protein and a fluorescence immune chromatography assay was established for the detection of rubella virus IgM antibody.Methods A lateral-flow（LF） device using the luminescent lanthanide nanoparticles as reporter was applied to detect rubella virus IgM antibody in the plasma.The device was made of a reaction well and a lateral-flow strip.Results Using ELISA as reference,the sensitivity,specificity and accuracy were 77.2%,82.3%,79.3%,in which the sensitivity and compliance rate are better than the colloidal gold immune chromatography assay.While the specificity was similar with colloidal gold chromatography assay.Conclusion The fluorescence immune chromatography assay is simple,rapid,high sensitive,overcoming the complicated operation,long time,high cost in the current rubella virus IgM detection,with potential application value.