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Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers
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摘要 Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers. Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第33期3107-3115,共9页 中国神经再生研究(英文版)
基金 supported by the National Natural Science Foundation of China,No.30600099(FD)
关键词 neural regeneration AMYLOID-BETA Alzheimer's disease OLIGOMER single-domain antibody phagedisplay antibody library construction ALPHA-SYNUCLEIN Parkinson's disease humanized antibody immunotherapy grants-supported paper NEUROREGENERATION neural regeneration amyloid-beta Alzheimer's disease oligomer single-domain antibody phagedisplay antibody library construction alpha-synuclein Parkinson's disease humanized antibody immunotherapy grants-supported paper neuroregeneration
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