摘要
旨在对食管鳞癌(ESCC)中miR-21调控细胞增殖和侵袭的机制进行探讨。平板克隆试验和伤口愈合体外试验发现,miR-21过表达可促进细胞增殖和迁移,相反,沉默miR-21可抑制细胞增殖和迁移。同时,Western blotting试验发现,miR-21过表达的Eca109细胞中p-ERK1/2表达上调,而在miR-21抑制的Eca109细胞中表达下调。试验结果提示miR-21可能通过激活ERK1/2/MAPK信号通路促进ESCC细胞增殖和侵袭,而且miR-21是在转录后水平正调控ERK1/2/MAPK信号通路。
It was to analyse the underlying mechanism of how miR-21 promotes proliferation and invasion in ESCC. Both plate colony formation and wound-healing assays showed that proliferation and migration of ESCC cell line Eca109 were increased in miR-21 mimics group and decreased in miR-21 inhibitor group after transfection into Eca109 cells with miR-21 mimics, miR-21 inhibitor and scramble sequence, respectively. Meanwhile, Western blotting results showed p-ERK1/2 expression was elevated in miR-21 mimics group, whereas decreased in miR-21 inhibitor group. The findings demonstrated that miR-21 promotes the proliferation and migration of Eca109 cell through activating ERK1/2/MAPK pathway;miR-21 can positively regulate and control the ERK1/2/MAPK signaling pathway at post-transcriptional level in Eca109 cells.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第12期189-193,共5页
Biotechnology Bulletin
基金
新疆自治区科技厅面上项目(2012211A080)
