SPE-HPLC法测定复方紫草油中β,β'-二甲基丙烯酰阿卡宁的含量

Determination of β,β'-Dimethyl-acry-lalkannin in Compound Zicao Oils by SPE-HPLC

目的:建立测定复方紫草油中β,β'-二甲基丙烯酰阿卡宁含量的方法。方法:采用Zorbax Eclipse XDB-C_(18)(150 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-水-甲酸(700:300:0.5),流速:1.0 ml·min^(-1),检测波长:275 nm,柱温:25℃,进样量:10μl。结果:β,β'-二甲基丙烯酰阿卡宁在5.2~104.0μg·ml^(-1)浓度范围内有良好的线性关系(r=0.999 8),平均加样回收率为100.2%,RSD为1.23%(n=6)。结论:此方法操作简便、专属性强、灵敏度高、重复性好,可用于该制剂的质量控制。

Objective：To establish a method for the determination of β,β-dimethyl-acry-lalkannin in compound Zicao oils.Method：A Zorbax Eclipse XDB-C18（150 mm×4.6 mm,5 μm） column was used,the mobile phase consisted of acetonitrile-water-formic acid（700：300：0.5） at the flow rate of 1.0 ml？min-1,the UV detector was set at 275 nm,the column temperature was 25℃,and injection volume was 10 μl.Result：The linear range of β,β’-dimethyl-acry-lalkannin was 5.2-104.0 μg？ml-1（r=0.999 8）.The average recovery was 100.2%（RSD = 1.23%,n =6）.Conclusion：The method is simple,specific,sensitive and reproducible,and can be applied in the quantitative determination of p,p’-dimethyl-acry-lalkannin in compound Zicao oils.