超氧阴离子自由基电化学分析的新进展

Progress on Electrochemical Determination of Superoxide Anion

超氧阴离子自由基(O2·-)是分子氧在生物体内氧化还原反应中产生的活性中间体,其动态变化可以提供丰富的生理、病理信息。因此,实时、在体检测O2·-自由基的分析方法越来越受到人们的关注。电化学分析方法具有直观、简单、易微型化等优点,在O2·-自由基检测中得到了广泛的应用。本文从溶液/电极界面的设计入手,利用酶的直接电子传递,结合O2·-自由基的分析特性,简要评述了近几年电化学分析在细胞和活体内O2·-自由基检测方面的研究进展。

Superoxide anion （ O2·-） is the primary species of the reactive oxygen species （ ROS）, resulting from the metabolism of O2·- in the living organisms. Its dynamic changes can provide broad implications in physiological and pathological conditions. Therefore, the analytical methods that can determine O2·- real-time and in vivo are receiving more and more attention. In light of these requirements, electrochemical approaches are the most promising candidate technologies in analysis of O2·- due to their simplicity, direct measurements, and ease of miniaturation. This review summarizes the recent progress on electrochemical analysis of O2·- taking into consideration toward the key issues： design of the electrolyte/electrode interface, direct electron transfer of enzymes, and applications in live cells and animals.