甘草提取物诱导胃癌MGC-803细胞凋亡的初步研究

Apoptosis of Human Gastric Cancer Cell Line MGC-803 Induced by Glycyrrhiza Uralensis Extract

目的 :研究甘草提取物诱导胃癌MGC 80 3细胞发生凋亡。方法 :用荧光双染、琼脂糖凝胶电泳、流式细胞仪等方法 ,观察甘草提取物处理MGC 80 3细胞后细胞形态和染色质DNA等的变化 ,以双重免疫标记法和免疫组化法检测处理前后 p53基因表达的变化。结果 :激光扫描共聚焦显微镜观察到处于不同凋亡进程中的细胞 ;流式细胞仪检测到显著的凋亡峰 ,且凋亡百分率呈浓度、时间依赖性 ;高浓度甘草提取物处理的MGC 80 3细胞的DNA琼脂糖凝胶电泳呈现典型的“ladder” ;甘草提取物对p53基因表达没有影响。结论 :甘草通过p53非依赖途径诱导MGC 80 3细胞凋亡 ,可望作为一种新的细胞凋亡诱导剂用于胃癌的治疗。

To study the apoptosis in human gastric cancer cell MGC-803 induced by the extract of glycyrrhiza uralensis Fisch. Methods: Apoptosis was detected by laser scanning confocal microscope, agarose gel electrophoresis and flow cytometry. The p53 gene expression was analyzed by double immunolabelling and immunohistochemistry technique. Results: Chromatin condensation of different phases of apoptotic cells were observed by laser scanning confocal microscope, the percentage of apoptosis determined by flow cytometry was time- and dose-dependent, agarose gel electrophoresis of DNA exhibited obvious “ladder”in the cells treated with higher concentration of the extract of glycyrrhiza uralensis. The p53 gene expression was unchanged after treatment. Conclusion: Glycyrrhiza can induce human gastric cancer cell line MGC-803 to apoptosis by p53-independent pathway and can be used as a potential, natural apoptosis-inducing agent for gastric cancer therapy.