HPLC-MS/MS法测定柴葛解肌汤中柴胡皂苷a、b2的含量

Determination of Saikosaponin a and Saikosaponin b2 in Chaige Jieji Decoction by HPLC-MS/MS

目的建立测定柴葛解肌汤中I型柴胡皂苷a与Ⅱ型柴胡皂苷b2的HPLC—MS／MS方法。方法采用Hypersil GOLDaQ色谱柱（150mm×2．1mm，3txm），乙腈-0．1％甲酸溶液为流动相，流速为300μL.min^-1，柱温：25℃，以液相色谱分离、电喷雾离子化串联进行检测。结果柴胡皂苷a、b2分别在44．8-672ng·mL～、47．2～708ng·mL。线性关系良好（r=0．9992、r=0．9993），平均回收率分别为98．53％和99．47％，RSD分别为2．01％和2．08％（n=6）。结论该方法快速简便、准确、灵敏度高，可用于柴葛解肌汤中柴胡皂苷a、b2的含量测定。

Objective To establish an HPLC-MS /MS method for the determination of saikosaponin a and saikosaponin b2 in Chaige Jieji decoction. Methods The separation was performed by using HPLC method with Hypersil GOLD aQ （150 mm ×2.1 mm, 3 μm） as chromatographic column. The mobile phase consisted of acetonitrile -0.1% formic acid and the flow rate was 300 μL.min^-1, the column temperature was set at 25 ℃, and the detection was done by electrospray ionization mass spectrometry. Results The linear range was 44.8-672 ng.mL^-1 for saikosaponin a（r=0.9992）, and 47.2-708 ng.mL^-1 for saikosaponin b2（r=0.9993）. The average recovery rates of saikosaponin a and saikosaponin b2 were 98.53 %, 99.47 %, and RSD were 2.01%, 2.08 %（n=6） , respectively. Conclusion The established method is simple, quick, sensitive and accurate, can be used to determine the content of saikosaponin a and saikosaponin b2 in Chaige Jieji decoction.