促孕散治疗卵巢静止不孕奶牛血清微量元素与激素的相关性分析

The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility- promoting powder

为了探讨促孕散治疗卵巢静止不孕奶牛血清中生殖激素和微量元素含量的关系,分析其相关性与回归方程,试验利用ELISA法测定了促孕散治疗卵巢静止不孕奶牛前后血清中促卵泡激素(FSH)、促黄体激素(LH)、孕激素(P4)和雌二醇(E2)的浓度,以原子吸收分光光谱法测定血清铜(Cu)、铁(Fe)、锰(Mn)和锌(Zn)含量,统计分析4种激素和4种微量元素间的相关关系与回归方程。结果表明:Cu、Mn与P4呈正相关,Fe、Zn与P4呈负相关;Cu与E2呈负相关,Mn、Fe、Zn与E2呈正相关;Cu与FSH呈负相关,Mn、Fe、Zn与FSH呈正相关;Cu与LH呈负相关,Fe、Mn、Zn与LH呈正相关。说明卵巢静止不孕奶牛血清中微量元素含量对激素的分泌有一定影响。

To investigate the relationship between reproductive hormones and trace elements in serum of infertile dairy cattle with ovarian stationary treated with fertility -promoting powder, and analyze of the correlation and regression equation between reproductive hormones and trace elements. The enzyme - linked ：immunosorbent assay （ELISA） were used to detect the concentrations of follicle - stimulating hormone （ FSH）, luteinizing hormone （ LH）, progesterone （ P4 ） and estradiol （ E2 ） in serum of infertile dairy cattle with ovarian stationary treated with fertility - promoting powder, and the atomic absorption spectrometry was used to determine the contents of serum copper （Cu）, iron （Fe）, manganese （Mn） and zinc （Zn）. The statistical analysis was performed for the correlation and regression equation between the four kinds of hormones and the four kinds of trace elements. The results showed that P4 was positively correlated with Cu and Mn, and was negatively correlated with Fe and Zn; E2 was negatively correlated with Cu, and was positively correlated with Mn, Fe＇, and Zn; FSH was negatively correlated with Cu, and was positively correlated with Mn, Fe, and Zn; LH was negatively correlated with Cu, and was positively correlated with Mn, Fe, and Zn. The resuits indicate that the contents of trace elements have some effects on the secretion of the hormones in serum of infertile dairy cattle with ovarian stationary.