MicroRNA-17-92簇对K562细胞生物学特性的影响及机制初探

Effect of MicroRNA-17-92 Cluster on the Biological Characteristics of K562 Cells and Its Mechanisms

本研究旨在探讨microRNA-17-92对K562细胞生物学特性的影响。以K562细胞系为模型,采用lipofectin2000转染miR-17-92 mimic,应用Q-PCR方法鉴定基因表达水平,CCK-8细胞增殖检测试剂盒检测K562细胞增殖活性,Annexin V-FITC/PI标记检测K562细胞的凋亡;细胞经过乙醇固定后利用流式细胞仪检测K562细胞周期变化,Western blotting检测相关蛋白Crk的表达。结果显示:K562细胞系转染miR-17-92 mimic后miR-17-92表达增高;miR-17-92高表达后K562细胞的增殖能力增强、在细胞周期方面由G1期向S期细胞增加;同时,miR-17-92 mimic能够抑制乏血清诱导的K562细胞凋亡;Western blot检测显示miR-17-92 mimic促进Crk蛋白的表达。结论:miR-17-92可以促进K562细胞增殖,抑制其凋亡并调控细胞周期变化。

The objective of this study was to explore the effects of microRNA-17-92 on the biological characteristics of K562 cells.The expression of miR-17-92 in K562 cells transfected with miRNA-17-92 mimic was detected by real time PCR.The effect of microRNA-17-92 on K562 cell proliferation was detected by CCK-8 method.Apoptosis of K562 cells was detected by Annexin V-PI labeling.Cell cycle distribution was determined by using flow cytometry.Western blot was performed to determine the protein levels of Crk.The results indicated that the transfection with miR-17-92 mimic increased expression of mature miR-17-92 in K562 cells.Compared with control group,cell proliferation and cell amount in S-phase of miR-17-92 mimic transfected group significantly increased,cell apoptosis decreased.The expression of signal connector protein Crk was greatly up-regulated in miR-17-92-mimic-transfected K562 cells.It is concluded that miR-17-92 can promote proliferation,inhibit apoptosis and regulate the cell cycle of K562 cells.