HPLC法同时测定金银花及金芪降糖片中9种成分的含量

Simultaneous determination of nine components in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet by HPLC

[目的】建立高效液相色谱（HPLC）法同时测定金银花及金芪降糖片中新绿原酸、隐绿原酸、绿原酸、咖啡酸、木犀草苷、芦丁、金丝桃苷、异绿原酸A、异绿原酸C9种成分的含量。【方法】色谱柱为WaterssymmetryshieldTMRPl8柱（250mmx4．6mm，5Ixm），流动相为乙腈（A）-水（0．1％磷酸，B），线性梯度洗脱：0-5min，10％～13％A；5—20rain，13％～15％A；20－30min，15％～18％A；30--45min，18％～20％A；45～52min，20％-23％A；52--70min，23％A，体积流量1．0mlJmin，检测波长327nm；柱温25℃。[结果】9种成分均能达到基线分离，线性关系良好，加样回收率在95％～105％。在该方法下测定了16批金银花药材、11批金芪降糖片中9种成分的含量。【结论】该法专属性强、灵敏度高、重复性好、简便易行，可用于同时测定金银花及金芪降糖片中新绿原酸、隐绿原酸、绿原酸、咖啡酸、木犀草苷、芦丁、金丝桃苷、异绿原酸A、异绿原酸C9种成分的含量，可为金芪降糖片的现代制剂研究和质量控制提供依据。

[Objective] To develop a HPLC method for simultaneous determination of neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, caffeic acid, eynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenie acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. [Methods] The chromatographic conditions were follows： Waters Symmetry Shield TM RP18 column （250 mm× 4.6 mm, 5 μm）, acetonitrile （A） and water （ 0.1% phosphoric acid, B） as mobile phases for gradient elution, 0-5 min, 10%-13% A, 5- 20 min, 13%-15% A;20-30 rain, 15%-18% A;30-45 min, 18%-20% A;45-52 min,20%-23% A;52-70 min,23% A; the flow rate being 1.0 mL/min. The detection wavelength was set at 327 nm and the column temperature was 25 ℃. [Results] The results showed that nine components were well separated and showed good linearity. The average recoveries were between 95%-105%. [Conclusion] This is a specific, sensitive, repeatable and simple method for simultaneous determining neochlorogenic acid, eryptochlorogenic acid, chlorogenic acid, eaffeie acid, cynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenic acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. The method can be available for the quality control of Jinqi Jiangtang tablet.