柑桔皮渣提取液对羟自由基的清除作用

SCAVENGING EFFECT OF EXTRACT FROM CITRUS PEEL ON OH

研究了柑、甜橙、梁平柚皮渣的水、乙醇、正丁醇、乙酸乙酯提取液对活性氧·OH的清除作用。方法 :以·OH清除剂 (甘露醇 )为对照 ,应用Fenton反应检测柑桔皮对·OH的清除作用。结果 :柑、甜橙、梁平柚皮渣的水提液IC50 分别相当于 3 .7,5 .6 ,6 .5mg(鲜皮 ) /ml。柑、梁平柚皮渣 70 %乙醇提取液的IC50 分别相当于 36 .2 ,5 0 .0mg(鲜皮 ) /ml,柑、甜橙、梁平柚皮渣的正丁醇提取液的IC50 分别相当于为 5 .2 ,4.4,9.2mg(鲜皮 ) /ml,柑、甜橙、梁平柚皮渣的乙酸乙酯提取液的IC50 分别相当于为 5 .4,4.6 ,30 .0mg(鲜皮 ) /ml。结论 :柑、甜橙、梁平柚皮渣的水提液对·OH具有较强的清除作用 ,柑、甜橙的正丁醇和乙酸乙酯提取液也具有较强的清除效果。而柑、甜橙、梁平柚皮渣的乙醇提取液 ,以及梁平柚正丁醇。

Fenton reaction was used to test the scavenging effects of water, ethanol, n-butanol and ethyl acetate extracts from the peel of penggan ( Citrus nobilis), sweet orange ( C.sinensis) and pomelo ( C.grandis) for hydroxyl free radicals with mannitol as the control. The IC 50 of water,70% ethanol, n-butanol and ethyl acetate extracts from Penggan,sweet orange and pomelo peel was 3.7,5.6 and 6.5; 36.2 and 50.0; 5.2,4.4 and 9.2; and 5.4,4.6 and 30.0mg(fresh peel)/ml, respectively. It is , therefore, concluded that the water extract from all the three Citrus species and the n-butanol and ethyl acetate extracts from Penggan and sweet orange can be used to scavenge hydroxyl free radicals, and the scavenging ability of other extracts in the experiment is poor.