黄芩总黄酮和栀子总环烯醚萜对含药小鼠血清体外抗病毒作用

Total flavonoids from Scutellaria baicalensis and total iridoid glycosides from Gardenia jasminoides inhibit influenza H1N1 in vitro

目的探讨黄芩总黄酮、栀子总环烯醚萜苷及其组合物含药小鼠血清体外抗甲型流感病毒(H1N1)的作用。方法采用细胞病变法测定黄芩总黄酮、栀子总环烯醚萜苷及其组合物含药血清对人肺腺癌细胞(A549)的最大无毒浓度和H1N1对A549细胞的半数组织感染量(TCID50);采用四甲基偶氮唑蓝比色法检测黄芩总黄酮、栀子总环烯醚萜苷及其组合物含药血清对H1N1的体外抑制作用和直接杀灭作用。结果黄芩总黄酮、栀子总环烯醚萜苷及其组合物含药血清对A549细胞的TC0均为12.5%;H1N1对A549细胞的TCID50为10-2.9/0.1 mL;黄芩总黄酮含药血清12.5%、黄芩总黄酮和栀子总环烯醚萜苷组合物含药血清6.25%和12.5%,分别对20倍量TCID50H1N1的细胞内增殖具有明显的抑制作用,且对H1N1具有直接的杀灭作用;黄芩总黄酮、栀子总环烯醚萜苷单用的药效作用弱于组合使用。结论黄芩总黄酮和栀子总环烯醚萜苷组合物具有明显的抗病毒效应,其作用机制可能是抑制病毒在细胞内的增殖和对病毒的直接杀灭作用。

AIM To study the anti-influenza A virus （ H1 N1 ） effect of the serum containing total flavonoids from Scutellaria baicalensis （TFSB）, the serum containing total iridoid glycosides from Gardenia Jasminoides （TIGGJ） and the serum containing the mixture of both in vitro. METHODS The TC0 of the serum containing drug to A549 cells and the TCIDs0 of H1N1 to A549 cells were determined by CPE assay. The inhibitory and direct killing effects of the serum containing drug to H1N1 in vitro were determined by MTT colorimetric assay. RE SULTS The TC0 of the serum containing TFSB, the serum containing TIGGJ and the serum containing the mixture of both to A549 cells was 12. 5% , respectively. The TCIDs0 of H1N1 to A549 cells was 10^-29/0. 1 mL. 12.5% serum containing TFSB, 6. 25% and 12.5% serum containing the mixture of both significantly inhibited the multi plication of H1N1 in A549 cells, while they also showed the direct killing action to H1N1. The drug action of TFSB, TIGGJ was less than that of the mixture of both. CONCLUSION The anti-virus effect of the mixture of both is related to the inhibitory multiplication of virus in cells and the direct killing action to virus