发光二极管光质对念珠藻葛仙米生长及生理生化特性的影响

EFFECT OF LIGHT-EMITTING DIODE LIGHT QUALITY ON THE GROWTH AND PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF NOSTOC SPHAEROIDES

利用发光二极管(LED)作为光源,以冷百荧光灯光作为对照,研究不同光质[红光637 nm、绿光529 nm、蓝光453 nm、白光(400—700)nm]对念珠藻葛仙米生长和生理生化特性的影响。结果表明:在培养前期,红光促进藻蓝蛋白合成,而藻红蛋白合成受抑制;蓝光和绿光则促进藻蓝蛋白合成。在培养后期,红光处理有利于叶绿素a和类胡萝卜素积累,其含量分别达到干重的1.33%和0.24%;绿光、白光和冷白荧光培养物的相应色素的含量均约占1.0%和0.16%;蓝光培养物的相应色素含量分别仅为0.45%和0.11%。红光培养物的氨基酸含量达干重的23.1%,是对照的1.58倍。除蓝光外其他光质对还原糖的含量影响无显著差异。在培养过程中LED白光和冷白荧光培养物的平均相对生长速率分别约为其他色光培养物的1.3和1.5倍。

The influence of different Light-Emitting Diode （LED） wavelengths （red 637 nm, green 529 nm, blue 453 nm and white light, set cool white fluorescence as control） on the growth and the physiological and biochemical characte-ristics of Nostoc sphaeroides K＆#252;tzing were studied. Results showed that in the initial growth stage, red light promoted the synthesis of phycocyanin and inhibited the phycoerythrin, and the former was also increased by blue and green lights. In the late growth stage, the contents of chlorophyll a and carotenoids in the colonies which cultured under red light accounted for 1.33%and 0.24%of the dry weight, while they were about 1.0%and 0.16%respectively in green, white, or cool white fluorescence cultures, and 0.45%and 0.11%respectively in blue light culture. The content of total amino acids in red light culture was 23.1%in dry weight, which was 1.58 times of the control group. There was no significant difference between the contents of reducing sugars in the cultures under different light wavelengths except blue light. The mean specific growth rates of the white light culture and the cool white fluorescence cultures were about 1.3 and 1.5 times of those in other monochromatic light cultures, respectively.