摘要
目的:观察姜黄素对β-淀粉样前体蛋白swe基因/早老蛋白1dE9基因(APPswe/PSEN1dE9)双转基因小鼠海马神经元B类Ⅰ型清道夫受体(scavenger receptor class B typeⅠ,SR-BⅠ)和三磷酸腺苷结合盒A1(ATP-binding cassette transporter,ABCA1)表达的影响,探讨姜黄素在阿尔茨海默病(Alzheimer’s disease,AD)防治中的机制。方法:将10只6月龄的APPswe/PSEN1dE9双转基因小鼠随机分为对照组和姜黄素饲喂组,每组5只。姜黄素饲喂组每天饲喂500 ppm姜黄素,6个月后采用免疫组化法检测小鼠海马神经元SR-BⅠ和ABCA1表达的变化。结果:与对照组相比,姜黄素饲喂组小鼠海马神经元ABCA1表达明显增加(t=-10.805,P=0.000),但2组小鼠海马神经元中均未见SR-BⅠ表达。结论:姜黄素能提高APPswe/PSEN1dE9双转基因小鼠海马神经元ABCA1的表达,而SR-BⅠ在神经元中未见表达。
Objective :To observe the effect of curcumin on expression of scavenger receptor class B type I (SR-B I ) and ATP-bind- ing cassette transporter 1 (ABCA1) in neurons of hippocampus of APPswe/PSENldE9 double transgenic mice and to investigate the mechanism of curcumin in the prevention and treatment of Alzheimer's disease. Methods:Ten six-month-old APPswe/PSEN l dE9 double transgenic mice were randomly divided into control group and curcumin group. Each group had 5 mice. Mice in curcumin group were fed curcumin 500 ppm every day. Six months later,changes of the expression of SR-B I and ABCA1 in neurons of hippicampus were detected by immunohistochemistry. Results :Expression of ABCA1 in neurons of hippocampus of mice in curcumin group was increased significantly compared with that of control group (t=-10.805,P=0.000) while expression of SR-B I was not detected in both groups. Conclusions:Curcumin can induce expression of ABCA1 in neurons of hippoeampus of APPswe/PSENldE9 double transgenie mice while expressions of SR-B I can not be detected in neurons.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2014年第2期141-145,共5页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:81271426、30973154、81100948)