利用RAPD技术分析兰属(Cymbidium)品种间的亲缘关系

ANALYSIS OF RELATIONSHIP AMONG CYMBIDIUM CULTIVARS USING RAPD

本文利用RAPD技术来检测兰属 13个品种间的亲缘关系 .通过对 5 5种 10个碱基随机引物的筛选 ,其中 4种引物能得到重复性、稳定性较高扩增产物 ,四种引物共扩增出 93条多态性带 ,多态率为 10 0 % .根据RAPD标记进行邻体聚类分析 ,表明兰属各种间的亲缘关系与形态学分类结果不完全一致 .实验结果认为RAPD技术可用于兰属植物的系统学研究 .图 2表 3参

RAPD technique was used to detect the relationships among 13 Cymbidium cultivars. Four 10-mer random primers that were screened from 55 primers obtained reproducible and constant amplified products,including 93 polymorphic markers. The rate of polymorphism was 100%. The results of neighbor-joining cluster analysis had some differences with those of morphological taxonomic study. It is shown that RAPD is a suitable method in taxonomic study on Cymbidium. Fig 2, Tab 3, Ref 13