结直肠腺癌KIAA1199基因表达与临床病理学相关性

Associations of KIAA1199 expression with clinicopathological features and prognosis in colorectal adenocarcinoma patients

目的探讨KIAA1199基因在结直肠腺癌中的表达与临床病理特征及预后的相关性及临床意义。方法采用实时定量PCR法(qPCR)检测手术切除结直肠腺癌及癌旁正常组织KIAA1199 mRNA表达;采用免疫组织化学染色(IHC)检测结直肠腺癌组织蜡块KIAA1199蛋白原位表达;分别分析KIAA1199 mRNA、蛋白表达水平与患者临床病理特征及预后生存的相关性。结果结直肠腺癌组织KIAA1199 mRNA水平(2-△△Ct=42.410 6±1.060 5)明显高于癌旁正常组织(2-△△Ct=1)(P<0.01),结直肠腺癌组织KIAA1199 mRNA水平与患者年龄、淋巴结及远处转移、临床分期呈正相关(P<0.01),与患者生存期呈负相关(P<0.01);结直肠腺癌组织KIAA1199蛋白表达水平[83.00%(83/100)]明显高于癌旁正常组织(阴性)(P<0.01),结直肠腺癌组织KIAA1199蛋白表达水平与原发灶浸润深度及临床分期呈正相关(P<0.01),与患者生存期呈负相关(P<0.01)。结论 KIAA1199基因可作为一种较有价值的评估结直肠腺癌病程进展及预后的标志物。

Objective To investigate correlations between KIAA1199 expression and clinicopathological features and prognosis in colorectal adenocarcinoma patients. Methods Real-time quantitative polymerase chain reaction（qPCR）was applied to detect the KIAA1199 mRNA expression levels in resected samaples of colorectal adenocarcinoma and the nor- mal tissue adjacent to the carcinoma. Immunohistochemistry（IHC） was used to detect the KIAA1199 expression levels in paraffin blocks from the patients with colorectal adenocarcinoma. Kaplan-Meier survival curve was applied to analyze the correlation between KIAAl199 expression and survival of colorectal adenocarcinoma patients. Results KIAA1199 mRNA expression level of colorectal adenocarcinoma（2-A Act = 42. 410 6 _＋ 1. 060 5 ）was significantly higher than that of the normal tissue adjacent to the carcinoma（2-a^ct = 1 ） （P 〈0. 01 ） and was correlated with the age of the patients, lymph node and tumor-node-metastasis（TNM） stage（ P 〈 0. 01 ）and negatively correlated with survival time（ P 〈0. 01 ）. IHC results showed that KIAA1199 protein expression levels of colorectal adenocarcinoma（ 83/100,83.00% ）was signif- icantly higher than that of the normal tissue adjacent to the carcinoma（ P 〈 0. 01 ）and was positively correlated with the invasion depth of primary tumor and TNM stage（P 〈 0. 01 ）, and negatively correlated with survival time of the patients （ P 〈 0. 01 ）. Conclusion KIAA1199 can be considered as a valuable marker for the assessment of progression and prog- nosis of colorectal adenocarcinoma.