摘要
植物干旱诱导蛋白是植物体在遭遇干旱胁迫或缺水胁迫时被诱导表达或者表达量被上调的一类蛋白,通过直接作用或者调节其他基因的表达来使植物适应干旱逆境(Desclos et al.,2008;Urao et al.,1994)。这类蛋白在功能上分为2类:其一为功能蛋白,在细胞内直接参与保护作用。
Drought-induced proteins,which are induced or up-regulated by water deficient stress,play an important role in drought resistance process of plants. In this research,full length cDNA of PwWDS1( GenBank accession number KJ526353),a drought induced gene,was obtained by RACE PCR method based on the full length cDNA library of Picea wilsonii. Several software and tools such as Expasy,DNAMAN,ClustalX and MEGA were used to analyze the physical and chemical properties of PwWDS1. Then expression pattern of PwWDS1 was identified by RT-qPCR and semiquantitative RT-PCR under drought,salt stress,abscisic acid( ABA) and ethrel treatments. We found that the full length cDNA of PwWDS1 was 1 077 bp encoding a protein which consists of 207 amino acids residues. The theoretical molecular weight of PwWDS1 was 22. 76 kDa with isoeletric point of 5. 02. Sequence analysis indicated that C terminal of PwWDS1 had a conserved ABA / WDS domain and N terminal had a strong hydrophilic property. RT-qPCR and semi-quantitative RT-PCR showed that PwWDS1 was mainly expressed in needles of P. wilsonii and the expression level of PwWDS1 increased with the growth of cotyledon during seed germination. The PwWDS1 expression was up-regulated by drought,salt stress,ethrel and ABA treatments. PwWDS1 was expressed highly in 6 h under drought and ABA treatments. Under salt and ethrel treatments,PwWDS1 was expressed most in 12 h. These results indicated that PwWDS1 would play a role in response to signals of abiotic stresses and plant hormones in P. wilsonii.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2014年第4期129-136,共8页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(31270663)
国家转基因生物新品种培育科技重大专项(2011ZX08009-003-002)
关键词
青杄
WDS
生物信息学分析
组织表达
胁迫响应
Picea wilsonii
water deficiency stress(WDS)
bioinformatic analysis
tissue expression
stress response