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青杄干旱诱导基因PwWDS1的cDNA分离与表达分析 被引量:2

Isolation and Expression Analysis of PwWDS1 in Picea wilsonii
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摘要 植物干旱诱导蛋白是植物体在遭遇干旱胁迫或缺水胁迫时被诱导表达或者表达量被上调的一类蛋白,通过直接作用或者调节其他基因的表达来使植物适应干旱逆境(Desclos et al.,2008;Urao et al.,1994)。这类蛋白在功能上分为2类:其一为功能蛋白,在细胞内直接参与保护作用。 Drought-induced proteins,which are induced or up-regulated by water deficient stress,play an important role in drought resistance process of plants. In this research,full length cDNA of PwWDS1( GenBank accession number KJ526353),a drought induced gene,was obtained by RACE PCR method based on the full length cDNA library of Picea wilsonii. Several software and tools such as Expasy,DNAMAN,ClustalX and MEGA were used to analyze the physical and chemical properties of PwWDS1. Then expression pattern of PwWDS1 was identified by RT-qPCR and semiquantitative RT-PCR under drought,salt stress,abscisic acid( ABA) and ethrel treatments. We found that the full length cDNA of PwWDS1 was 1 077 bp encoding a protein which consists of 207 amino acids residues. The theoretical molecular weight of PwWDS1 was 22. 76 kDa with isoeletric point of 5. 02. Sequence analysis indicated that C terminal of PwWDS1 had a conserved ABA / WDS domain and N terminal had a strong hydrophilic property. RT-qPCR and semi-quantitative RT-PCR showed that PwWDS1 was mainly expressed in needles of P. wilsonii and the expression level of PwWDS1 increased with the growth of cotyledon during seed germination. The PwWDS1 expression was up-regulated by drought,salt stress,ethrel and ABA treatments. PwWDS1 was expressed highly in 6 h under drought and ABA treatments. Under salt and ethrel treatments,PwWDS1 was expressed most in 12 h. These results indicated that PwWDS1 would play a role in response to signals of abiotic stresses and plant hormones in P. wilsonii.
出处 《林业科学》 EI CAS CSCD 北大核心 2014年第4期129-136,共8页 Scientia Silvae Sinicae
基金 国家自然科学基金项目(31270663) 国家转基因生物新品种培育科技重大专项(2011ZX08009-003-002)
关键词 青杄 WDS 生物信息学分析 组织表达 胁迫响应 Picea wilsonii water deficiency stress(WDS) bioinformatic analysis tissue expression stress response
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  • 1许家春,邵海燕,李殿波.优良绿化树种青扦云杉引种栽培技术[J].中国林副特产,2004(3):24-25. 被引量:11
  • 2杨映根,桂耀林,唐巍,赵燃,郭仲琛.青杄愈伤组织在继代培养中的分化能力及染色体稳定性研究[J].Acta Botanica Sinica,1994,36(12):934-939. 被引量:22
  • 3周祥明,张冰玉,苏晓华,王大海,黄秦军,张香华,张志毅.美洲黑杨雄性花芽cDNA克隆测序及表达序列标签(ESTs)特性分析[J].林业科学,2006,42(11):37-41. 被引量:4
  • 4内蒙古通辽市植保植检站,内蒙古通辽028000,郭九峰,孙国琴,沈传进,乔惠蕾,贾利敏,郭志杰.沙冬青cDNA文库的构建和EST分析[J].华北农学报,2007,22(4):37-41. 被引量:16
  • 5庄道德.云杉喷灌全光育苗试验.四川林业科技,1981(4):29-30.
  • 6Kong L, Wang M, Wang Q, et al. Protein phosphatases 1 and 2A and the regulation of calcium uptake and pollen tube development in Picea wilsonii. Tree Physiol, 2006, 26 ( 8 ) : 1001-1012.
  • 7Sheng X, Hu Z, Lu H, et al. Roles of the ubiquitin/proteasome pathway in pollen tube growth with emphasis on MG132-induced alterations in ultrastructure, cytoskeleton, and cell wall components. Plant Physiol, 2006, 141 ( 4 ) : 1578-1590.
  • 8Sheng X, Dang X, Zhang S, et al. Mitochondrial dynamics and its responds to proteasome defection during Picea wilsonii pollen tube development. Cell Biochem Funct, 2010, 28 ( 5 ) : 420-425.
  • 9Yu Y, Li Y, Li L, et al. Overexpression of PwTUA1, a pollen-specific tubulin gene, increases pollen tube elongation by altering thedistribution of alpha-tubulin and promoting vesicle transport. J Exp Bot, 2009, 60 ( 9 ) : 2737-2749.
  • 10Zheng M, Wang Q, Teng Y, et al. The speed of mitochondrial movement is regulated by the cytoskeleton and myosin in Picea wilsonii pollen tubes. Planta, 2010, 231 ( 4 ) : 779-791.

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