新型压电陶瓷材料钛酸铋钠的体外生物相容性研究

Biocompatibility research of a new Bi_(0.5)Na_(0.5)TiO_3 piezoelectric ceramic material in vitro

目的：通过体外实验对钛酸铋钠（ BNT）基无铅压电陶瓷进行生物相容性评价。方法：将BNT组、铌酸锂钠钾组、聚甲基丙烯酸甲酯组及空白对照组与人成骨样细胞进行复合培养，应用扫描电镜、四唑盐比色法观察材料对成骨样细胞形态及增殖的影响。结果：成骨样细胞在实验材料上可很好地黏附、增殖和生长。 BNT组在实验第1~9天光密度值均高于铌酸锂钠钾组、聚甲基丙烯酸甲酯组和空白对照组（P〈0.01）。结论：新型钛酸铋钠基无铅压电陶瓷初步显示了良好的体外生物相容性，有望成为一种新的生物材料。

Objective：To evaluate the biocompatibility of Bi0.5 Na0.5 TiO3-based（BNT） lead-free piezoceramics material in vitro. Methods：Human osteoblast-like cell lines were cultured with BNT, （ Lix Nay K1-x-y ） NbO3-based, polymethylmethacrylate, and blank control groups,the morphology and proliferation function of the osteoblast-like cells were observed by scanning electron microscopy and 3-（4, 5-Dimethylthiazol-2-yl ）-2, 5-diphenyltetrazolium bromide assay. Results：The osteoblast-like cell lines adhered, grew and proliferated normally and perfectly on the surface of the material. Compared with the （ Lix Nay K1-x-y ） NbO3-based,polymethylmethacrylate and blank control groups,BNT showed a higher optical density at 1,3,5,7,9 days（P〈0. 01）. Conclusions：The new piezoelectric ceramic seems to possess the good biocompatibility in vitro,it may be a new type of biological material.