逆转录病毒介导的HSV-TK基因系统对人胃癌细胞的转染及杀伤作用

Transfecting and killing effect of herpes simplex virus thymidine kinase (HSV-TK) gene on human gastric carcinoma in vitro

目的 :探讨逆转录病毒介导的单纯疱疹病毒胸苷激酶 (HSV- TK)基因系统对人胃癌细胞的转染及杀伤作用。方法 :应用 PA317细胞包装的逆转录病毒介导的 HSV - TK基因系统 ,在体外以逆转录病毒上清感染法将 TK基因导入 MKN2 8人胃癌细胞 ,并初步观察更昔洛韦 (GCV )对转染 TK基因的 MKN2 8胃癌细胞的杀伤作用。 结果 :TK基因可成功转导入MKN2 8细胞 ,且对细胞的生长状态无明显影响 ,但对 GCV的敏感性却显著增加 ;同时还观察到显著的“旁观者效应”。结论 :逆转录病毒介导的 HSV- TK系统对人胃癌细胞有较高的转染效率 ,GCV对转染阳性的胃癌细胞有显著的杀伤作用。

Objective: To study retrovirus (RV) mediated transduction of gastric carcinoma cells with the herpes simplex virus thymidine kinase (HSV tk) gene and the subsequent treatment with ganciclovir(GCV). Methods: The TK gene was transfected into human gastric carcinoma cell line MKN28 using HSV TK that packed with PA317 cell, the sensitivity of MKN28TK cells to GCV was examined in vitro . Results: The retroviral mediated HSV TK gene can be transfected to MKN28 cells. The growth rate of MKN28 cells transfected with HSV TK gene did not change. MKN28TK cells became significantly sensitive to GCV and had bystander effect. Conclusion: Transfection of gastric carcinoma with HSV TK has higher transfection efficiency. MKN28TK cells are significantly sensitive to GCV. [