凝血酶原基因G20210A变异在血栓栓塞症中的研究

Preliminary Investigation of Prothrombin Gene Variation (20210 G to A) in Chinese Patients with Thromboembolic Diseases

目的 研究凝血酶原基因Ｇ２０２１０Ａ变异在血栓栓塞症的发病情况。方法 ３０例深静脉血栓形成患者、３２例脑血栓患者和４０名健康献血者进行聚合酶链反应然后进行限制性内切酶Ｈｉｎｄ Ⅲ酶切、琼脂糖凝胶电泳分析。结果 患者和健康献血者的琼脂糖凝胶电泳结果均显示正常带型，即３４５ｂｐ一条带。结论 凝血酶原基因Ｇ２０２１０Ａ变异在我国的发生率很低，很可能象ＦＶ Ｌｅｉｄｅｎ变异引起的活化蛋白Ｃ抵抗一样存在着明显的种族差异。

Objective To investigate the role of prothrombin variant 20210 G to A in Chinese patients with thromboembolic diseases. Methods 30 patients with deep venous thrombosis (DVT), 32 cases of cerebrovascular ischemic disease and 40 healthy volunteers were detected by the poymerase chain reaction (PCR), and then digestion of restriction endonuclease Hind Ⅲ and analysis of agarose gel electrophoresis were arried it. Results The pictures of agarose gel analysis in all patients and healthy volunteers showed a normal DNA fragment band (345bp). Conclusion The incidence of prothrombin variant 20210 A to G in Chinese patients with thromboembolic diseases is as low as that in patients with APC resistance and FV Leiden mutation. It may reflect plausible pathogenic divergence in the diseases among different races.