摘要
目的 对 rh BMP- 2在诱导成骨中 Collagen I,II及碱性磷酸酶 (AL P)的表达进行研究 .方法 将含 rh BMP- 2 0 .5mg的松质骨载体植入 5 5只 BAL B/ c小鼠的右侧股部肌袋内 ,分别于术后 3～ 2 1d共 8个时间点取材 ,采用免疫组织化学方法对新生骨组织中的 I,II型胶原进行检测 ;对 AL P活性进行定量分析 ,观察 rh BMP- 2在诱导成骨中 Collagen I,II及AL P的表达情况 .结果 1II型胶原的出现是和成软骨、软骨细胞的出现相伴随的 ,但是 ,肥大、变性的软骨细胞并不表达 II型胶原 ;2作为成骨细胞成熟标志物的 I型胶原、AL P在软骨形成期即有表达 ,但是随着成骨细胞的出现 ,骨组织的形成 I型胶原仍表现为高表达 ,而 AL P的表达则呈下降趋势 .结论 在诱导成骨中 rh BMP- 2促进了 Collagen I,II及AL P的表达 .
AIM To study expression of collagen I, II and alkaline phosphatase in osteogenesis by rhBMP 2. METHODS Compositions of rhBMP 2 (0.5 mg) and cancellous bone carrier were implanted in the right thigh pouch of 55 BALB/c mice. In order to investigate expression of collagen I, II and alkaline phosphatase in osteogenesis by rhBMP 2, immunohistochemistry analysis was performed 3, 5, 7, 9, 11, 14, 17 and 21 days after the implantation. Meanwhile, alkaline phosphatase activity was measured 7, 14, 21 days after the implantation. RESULTS 1. The appearance of type II collagen was accompanied by that of chondroblasts and chondrocytes, but hypertrophic and degenerative chondrocytes did not express type II collagen. 2. Type I collagen and ALP, osteoblast differentiation markers, were expressed at the early stage of chondrogenesis, but type I collagen was still expressed at higher level, ALP decreased gradually with the appearance of osteoblasts and osteogenesis. CONCLUSION rhBMP 2 might stimulate expression of collagen I, II and alkaline phosphatase in osteogenesis.
出处
《第四军医大学学报》
北大核心
2001年第11期981-983,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金!资助项目 (3 960 0 14 8)
