反相高效液相色谱中流动相线性梯度洗脱条件下溶质保留时间预测的新方法

A New Method for Predicting Retention Time of Solutes Under Linear Gradient Elution Conditions in RP-HPLC

在计算溶质的梯度保留时间时 ,根据流动相在色谱柱内的分布规律 ,对溶质在色谱柱内的迁移距离和流动相梯度同时进行校正 ,从而建立了一种预测溶质线性梯度洗脱条件下保留时间的新方法。该方法在不同的仪器系统中 ,对于弱保留和强保留溶质在不同线性梯度洗脱条件下保留时间的预测 ,都具有良好的准确度。以 15种氨基酸和 8种苯的同系物为例 ,该方法对于弱保留溶质保留时间的预测 ,相对平均误差分别为 3 70 %和 4 90 % ,远小于文献方法得到的结果 (2 3 6 1%和 31 16 % ) ;对于强保留溶质保留时间的预测 ,相对平均误差分别为 0 2 1%和6 0 1% ,略小于文献方法得到的结果 (0 81%和 6 6 9% )。

A new method for predicting the retention time of solutes under linear gradient elution has been established In this method, the migration distance of solutes in column and the mobile phase gradient were modified simultaneously according to the distribution of mobile phase in column The prediction results of retention time of both weakly and strongly retentive solutes were accurate in different instrumental systems using this method In order to test and verify this method, the retention time of fifteen amino acids and eight homologous compounds of benzene were predicted The predicted results were compared with those predicted by the method in common use The relative average errors between predicted and experimental values were 3 70% and 4 90% for the weakly retentive solutes, far smaller than those obtained by the reference method (23 61% and 31 16%) For the strongly retentive solutes, the relative average errors were 0 21% and 6 01% respectively, slightly smaller than those obtained by the reference method (0 81% and 6 69%) It is seen that this new method has many advantages