摘要
本实验提纯了玉米矮花叶病毒 ( MDMV)的辅助成份—蛋白酶 ( HC- Pro) ,并制备了其抗血清。MDMV HC- Pro的分子量约为 57k D,提纯产量为 0 .3mg/ 10 0 g病叶。琼脂免疫双扩散测定证明MDMV HC- Pro抗血清的效价为 1∶ 16;微量免疫沉淀法测定时其效价为 1∶ 10 2 4。该抗血清只与MDMV HC- Pro有明显的反应 ,与健康玉米汁液和 MDMV无反应。MDMV HC- Pro抗血清可以专化性地抑制 HC- Pro的蚜传活性。
Maize dwarf mosaic virus (MDMV) helper component proteinase (HC Pro) was purified and the specific antiserum to it was prepared in this experiment. The molecular weight of MDMV HC Pro was ca. 57 kD and its yield was 0.3 mg/100 g diseased leaves. The titer of antiserum was 1/16 in agar gel double diffusion test and 1/1024 in microprecipitation test. The antiserum reacted strongly with MDMV HC Pro, but did not with MDMV and healthy maize extracts. It could inhibit transmission efficiency of virus by aphids specifically.
出处
《植物病理学报》
CAS
CSCD
北大核心
2001年第2期135-138,共4页
Acta Phytopathologica Sinica
基金
攀登计划! (85 - 31- 0 2 - 0 3)
