自发性腹膜炎腹水细菌潜生体形成及其生物学特性研究

Study on the biological characteristics of cryptic growth cells of ascites in patients with spontaneous bacterial peritonitis

目的 :观察自发性腹膜炎患者腹水中细菌潜生体 (CGC)的生物学特性。方法 :用细菌形态学检测方法观察腹水细菌 CGC的形成 ;用细菌显微培养技术、细菌遗传稳定性试验和最小抑菌浓度 (MIC)测定并观察CGC的生物学特点。结果 :自发性腹膜炎患者腹水中 CGC具有传代生长、繁殖方式多样、活动能力强和抗生素耐受力强的特点。结论 :自发性腹膜炎患者腹水 CGC有很强的繁殖能力、运动能力和抗生素耐受力 。

Objective:To investigate the biological characteristics of cryptic growth cells (CGC) of ascites in patients with spontaneous bacterial peritonitis (SBP).Methods:Bacterial morphological examination was employed to identify the CGC formation in the ascitic fluid.Microculture technique,bacterial genetic stability test and minimal inhibitory concentration were used to observe the biological characteristics of CGC.Results:It was found that CGC in ascites of SBP was characterized by its generative ability in succeeding generations with high activity,multistyle of proliferation and powerful tolerance towards antibiotics.Conclusions:The CGC possessed powerful aggressive ability.