胃癌组织ras基因突变及其与幽门螺杆菌感染的关系

Investigation of the Mutation of Ras Gene in Gastric Cancer and Their Relation to Helicobacter Pylori(HP)infection

目的 初步探讨胃癌组织中 ras基因突变与幽门螺杆菌 ( Helicobacter pylori,Hp)感染的关系。方法 43例胃癌组织新鲜标本及相应血清标本纳入研究。用 PCR- RFLP法测定 ras基因 1 2密码子的突变 ;用血清学方法检测 Hp的感染状况。结果 43例胃癌中有 2 8例存在 ras基因1 2密码子的突变 ,突变率为 65 .1 2 % ;43例胃癌中 ,Hp阳性 30例 ,阳性率为 69.77% ,其中 Cag A阳性 2 4例 ,阳性率为 80 % ;30例 Hp阳性的胃癌中 ,有 1 9例发生 ras基因 1 2密码子的突变 ,发生率为 63.33% ,1 3例 Hp阴性的胃癌中 ,有 9例发生 ras基因 1 2密码的突变 ,发生率为 69.2 3% ,二者比较无明显差异 ( P>0 .0 5 )。结论 ras基因 1 2密码子的突变可能与胃癌的发生有关 ,胃癌中ras基因 1 2密码子的突变与 Hp及 Cag A阳性的 Hp感染无明显相关性 。

Objective To investigate the mutation of ras gene and their relation to Helicobacter pylori (Hp) in fection. Methods The carcinomatous tissues and serum samples of 43 patients with gastric cancer were studied. The mutation of the NO.12 codon of ras gene was detected by PCR-RFLP. The status of Hp infection was determined by seriological tests. Results 28 of 43 cases had NO.12 codon mutation, its mutation rate was 65.12%. In the 43 patients with gastric cancer, Hp infection was confirmed in 30 patients, CagA + was found in 24 cases(80%). Among the 30 cases with Hp infection, 19(63.33%)presented with the NO.12 codon of ras gene mutation and among those without Hp infection, 9(69.23)%cases showed the NO.12 codon of ras gene mutation, there is no significant difference between them( P >0.05). Conclusion The mutation of the NO.12 codon of ras gene may related to gastric cancer and it hasn't significant relation to Hp infection.