摘要
目的 通过原代培养的正常及受H2 O2 损伤的肺泡Ⅱ型上皮细胞 (ATⅡcell) ,探讨异氟醚 (Iso)对肺泡Ⅱ型上皮细胞表面活性物质相关蛋白A(SP A)的影响。方法 肺泡Ⅱ型上皮细胞培养 32小时后 ,被分为六组 :对照组 (不加任何药物 )、0 2 8mmol/LIso组、2 8mmol/LIso组、75 μmol/LH2 O2 组、75 μmol/LH2 O2 +0 2 8mmol/LIso组和 75 μmol/LH2 O2 +2 8mmol/LIso组 ,继续培养 3小时。通过酶联免疫吸附法 (ELISA)检测Ⅱ型上皮细胞内和培养液中SP A含量。结果 Iso降低正常肺泡Ⅱ型上皮细胞内和培养液中SP A含量 ;经H2 O2 作用后 ,SP A含量亦显著减少 ,异氟醚增强H2 O2 的作用。结论 Iso可降低肺泡Ⅱ型上皮细胞合成SP A的功能 ,尤其是在过氧化环境中。
Objective To explore the effect of isoflurane (Iso) on pulmonary surfactant related protein(SP A) of the alveolar type Ⅱ cells cultured primary and injured by H 2O 2.Methods AT Ⅱ cells were isolated from adult rat lungs and used for the experiments after 32h in primary culture and randomised to six groups:control group,0 28mmol/L Iso group,2 8mmol/L Iso group,75μmol/L H 2O 2 group,75μmol/L H 2O 2+0 28mmol/L Iso group and 75μmol/L H 2O 2+2 8mmol/L Iso group.Each group was continuously incubated for three hours after administration of Iso or/and H 2O 2.The intracellular SP A and the SP A of cultured medium were measured with an enzyme linked immunosorbent assay(ELISA).Results Iso significantly decreased SP A content of cultured medium and the intracellular,and augumented the decrease of SP A content induced by H 2O 2 in a dose dependent manner.Conclusion Isoflurane may decrease SP A synthesis of the alveolar type Ⅱ cells in vitro,and aggravate the damage of the alveolar tpye Ⅱ cells especially under peroxidation condition . [
出处
《临床麻醉学杂志》
CAS
CSCD
2001年第10期556-558,共3页
Journal of Clinical Anesthesiology