大肠杆菌感染对大鼠肺组织β-防御素-2基因表达的负相调节作用

Down-regulation of β-defensin-2 gene expression in E.coli-infected rat lung tissue

目的 :探讨细菌对大鼠 β -防御素 - 2 (rBD - 2 )基因的表达调控。 方法 :将大肠杆菌E .coliML -35p和绿脓杆菌ATCC 2 785 3大鼠气管内接种 ,于 2 4h后提取肺组织RNA ,用逆转录 -聚合酶链反应 (RT -PCR)法和Northernblot检测大鼠 β -防御素 - 2基因的表达变化。 结果 :气管内接种大肠杆菌 2 4h后使大鼠肺组织 β -防御素- 2mRNA的表达受到显著抑制 ,绿脓杆菌对其基因的表达却没有抑制作用。结论 :大肠杆菌感染可负相调节大鼠肺组织rBD - 2mRNA的表达。

AIM: To study the regulation of rat β-defensin-2 gene expression by bacteria.METHODS: E.coli ML-35 p and pseudomonas aeruginosa were injected into rat trachea. Total RNA was isolated from lung tissue after 24 h inoculation. RT-PCR and Northern blot were performed to detect β-defensin-2 ( rBD-2 ) mRNA expression.RESULTS: The expression of rBD-2 gene was inhibited in the lung tissue by E.coli infection, but not by pseudomonas aeruginosa. CONCLUSION: These results indicated that E.coli infection could down-regulate rBD-2 gene expression in the rat lung tissues.